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IB Biology C1.3.4 Photosynthetic pigment separation

IB Biology C1.3.4 Photosynthetic pigment separation
IB Biology syllabusBiology SL/HLFirst assessment 2025

Practise interpreting chromatography of leaf pigments, including solvent movement, pigment solubility, Rf values and chromatogram evidence.

How this is tested

  • describe how leaf or algal pigments are extracted and separated by chromatography
  • calculate Rf from pigment distance divided by solvent front distance
  • interpret chromatograms by comparing colours or Rf values with known pigments
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