In order to investigate the hypothesis that honeybees (Apis mellifera) have an instinct to forage for either nectar or pollen, but not both, researchers installed different feeders containing either nectar alone or pollen alone. They collected four different groups of honeybees (those arriving at the nectar feeders, arriving at the pollen feeders, departing from the nectar feeders and departing from the pollen feeders) and measured the abundance of eight peptides in their brains. The relative difference of these brain peptides was then calculated by subtracting the abundance in nectar foragers from the abundance in pollen foragers and is shown by the bars on the graphs below.