IB Biology SL C1.3.4 Photosynthetic pigment separation
IB Biology SL C1.3.4 Photosynthetic pigment separation
Practise interpreting chromatography of leaf pigments, including solvent movement, pigment solubility, Rf values and chromatogram evidence.
How this is tested
- describe how leaf or algal pigments are extracted and separated by chromatography
- calculate Rf from pigment distance divided by solvent front distance
- interpret chromatograms by comparing colours or Rf values with known pigments