Question 1
A student carried out an investigation to test cell respiration in yeast (Saccharomyces cerevisiae). Bottles were filled with 5 g of yeast and of water maintained at . A different mass of sucrose was added to each bottle: 5,15 and 30 g respectively.
Bottles were left for 15 minutes before attaching a standard balloon to the opening of each bottle. After 45 minutes, the circumference of each balloon was measured using a string. The volume of was calculated. Three trials per treatment were performed.
The diagram shows one trial, and the graph shows the mean of three trials.

Question 1(a)
State the independent variable.
Question 1(b)
Suggest a reason for waiting 15 minutes before attaching the balloons.
Question 1(c)
Outline a reason for keeping the temperature at .
Question 1(d)
Suggest a conclusion of this experiment.
Question 1(e)
Explain how the method could have been carried out more effectively by the student.







