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CAIE IGCSE Chemistry 12.3.3 Paper chromatography is used to

CAIE IGCSE Chemistry 12.3.3 Paper chromatography is used to
Cambridge IGCSE Chemistry 0620 syllabus for exams in 2026, 2027 and 20282026–2028

Practise separating colourless soluble mixtures by chromatography and explaining how locating agents make amino acid spots visible.

How this is tested

  • state that colourless amino acids can be separated by chromatography and detected with a locating agent
  • link a locating agent to coloured spots on a dried chromatogram, not to dissolving or preserving amino acids
  • use Rf after separation and detection to help identify colourless substances

Question 40

[Maximum number: 1]

Which statement about chromatography is correct?

A

It is not possible for two different substances to have the same RfR_{\mathrm{f}} value.

B

It is only possible to use chromatography on substances which have a colour.

C

It is possible to use chromatography on colourless substances using a locating agent.

D

The RfR_{\mathrm{f}} value of a substance = the distance travelled by the solvent  the distance travelled by the substance =\frac{\text { the distance travelled by the solvent }}{\text { the distance travelled by the substance }}