HL photosynthesis links light-dependent reactions to the Calvin cycle. Photosystems are pigment-protein arrays in thylakoid membranes; antenna pigments broaden absorption and pass energy to reaction-centre chlorophyll. PSII uses light to split water, supplying electrons, protons, and oxygen. Electron transport pumps protons into the thylakoid space, ATP synthase makes ATP by photophosphorylation, PSI re-excites electrons, and NADP is reduced. The Calvin cycle uses ATP and reduced NADP: Rubisco fixes CO2 to RuBP to form GP, GP is reduced to triose phosphate, most triose phosphate regenerates RuBP, and some becomes wider biomolecules.
- Trace energy: pigments -> reaction centres -> ATP and reduced NADP.
- Trace electrons/protons: water -> PSII -> carriers -> PSI -> NADP; proton gradient -> ATP synthase.
- Trace carbon: CO2 -> RuBP/GP -> triose phosphate -> RuBP regeneration and biomolecule synthesis.
- Trace exchange: light-dependent reactions supply ATP/reduced NADP; Calvin cycle returns ADP/NADP.