C1.3.16 (HL)—Triose phosphate synthesis

Triose phosphate forms when glycerate phosphate is reduced using ATP and reduced NADP during the light-independent reactions, connecting the mechanism to observable photosynthesis evidence.

Syllabus
First assessment 2025
Objective
C1.3.16
Level
HL

Exam analysis

Chance of appearing2%of analysed past papers
Latest appearanceNovember 2022
Most common paperPaper1
Typical marks1

Recent exam appearances

November 2022Paper1 ["HL"] · TZ031[ 1 ]C1.3.16 (HL)—Triose phosphate synthesis
May 2018Paper1 ["HL"] · TZ131[ 1 ]C1.3.16 (HL)—Triose phosphate synthesis
Practice this objective

Coverage 2018–2022 · Updated 15 Jul 2026

Reduction and RuBP regeneration

HL only
Calvin cycle middle steps.

Glycerate 3-phosphate (GP) is converted to triose phosphate (TP) using ATP and reduced NADP; most TP is then recycled to regenerate RuBP using ATP.

ATP supplies energy and reduced NADP supplies hydrogen/electrons for GP reduction. Regenerating the five-carbon CO₂ acceptor RuBP allows carbon fixation to continue.

For every six TP produced, five TP molecules are rearranged using ATP to form three RuBP molecules. Thus five-sixths of TP is recycled when glucose is treated as the photosynthesis product; one-sixth is available for product synthesis.

Three fixed CO₂ yield six TP after reduction: five TP regenerate three RuBP, while the carbon from one TP is the net gain. Two such net TP gains can supply the six carbons needed for one glucose.

The individual regeneration reactions are not required. Do not imply that each turn releases glucose or that all TP leaves the cycle.

Triose phosphate synthesis

HL only

Assessment in practice

1 marks
How it is assessed

This objective is assessed through multiple choice.

What earns marks

Build the answer around this relationship: Glycerate 3-phosphate is reduced to triose phosphate in the Calvin cycle.

Representative question

Question 1

[Maximum number: 1]

What occurs in the light-independent reactions of photosynthesis?

A

Glycerate 3-phosphate is reduced to triose phosphate.

B

Ribulose bisphosphate is regenerated using reduced NADP.

C

Ribulose bisphosphate is oxidized to two molecules of glycerate 3-phosphate.

D

Both ATP and NADP are used to produce triose phosphate.

Trace Photosynthesis Mechanism

HL only

HL photosynthesis links light-dependent reactions to the Calvin cycle. Photosystems are pigment-protein arrays in thylakoid membranes; antenna pigments broaden absorption and pass energy to reaction-centre chlorophyll. PSII uses light to split water, supplying electrons, protons, and oxygen. Electron transport pumps protons into the thylakoid space, ATP synthase makes ATP by photophosphorylation, PSI re-excites electrons, and NADP is reduced. The Calvin cycle uses ATP and reduced NADP: Rubisco fixes CO2 to RuBP to form GP, GP is reduced to triose phosphate, most triose phosphate regenerates RuBP, and some becomes wider biomolecules.

  • Trace energy: pigments -> reaction centres -> ATP and reduced NADP.
  • Trace electrons/protons: water -> PSII -> carriers -> PSI -> NADP; proton gradient -> ATP synthase.
  • Trace carbon: CO2 -> RuBP/GP -> triose phosphate -> RuBP regeneration and biomolecule synthesis.
  • Trace exchange: light-dependent reactions supply ATP/reduced NADP; Calvin cycle returns ADP/NADP.

Concept essentials

  • Glycerate 3-phosphate is reduced to triose phosphate in the Calvin cycle.
  • ATP supplies energy for triose phosphate synthesis.
  • Reduced NADP supplies reducing power for the reduction step.
  • Triose phosphate can contribute to carbohydrate synthesis or RuBP regeneration.