D3.2.20 (HL)—Recombinants

Recombinants have allele combinations different from parental combinations because crossing over reshuffles linked genes during meiosis in inheritance problems in inheritance problems.

Syllabus
First assessment 2025
Objective
D3.2.20
Level
HL

Exam analysis

Chance of appearing5%of analysed past papers
Latest appearanceMay 2022
Most common paperPaper1
Typical marks1

Common command terms

  • Deduce
  • Identify

Recent exam appearances

May 2022Paper1 ["HL"] · TZ136[ 1 ]D3.2.20 (HL)—Recombinants
May 2021Paper1 ["HL"] · TZ136[ 1 ]D3.2.20 (HL)—Recombinants
November 2019Paper2 ["HL"] · TZ02(b)[ 1 ]D3.2.20 (HL)—Recombinants
November 2015Paper2 ["HL"] · TZ04(c)[ 1 ]D3.2.20 (HL)—Recombinants
November 2014Paper1 ["HL"] · TZ034[ 1 ]D3.2.20 (HL)—Recombinants
Practice this objective

Coverage 2012–2022 · Updated 16 Jul 2026

Use a Test Cross to Identify Recombinants

HL only

A recombinant carries a new allele combination relative to the parental chromosome combinations, produced by crossing over for linked genes or reassortment for unlinked genes.

Cross an individual heterozygous at both loci with an individual homozygous recessive at both. The recessive parent contributes only ab, so each offspring genotype and phenotype reveals the gamete made by the heterozygote.

Heterozygote gamete Test-cross offspring genotype Classification for AB/ab parent
AB AaBb Parental
ab aabb Parental
Ab Aabb Recombinant
aB aaBb Recombinant

If parental classes greatly exceed recombinant classes, the loci are linked; approximately 1:1:1:1 supports an unlinked or effectively 50%-recombining model.

Identify parental combinations from the cross, not from which phenotype looks 'normal'. Recombination frequency cannot exceed 50%.

Recombinants exam focus

HL only

Assessment in practice

1 marks
How it is assessed

This objective is assessed through multiple choice, commonly using Deduce / Identify.

Command terms

Deduce / Identify

What earns marks

Build the answer around this relationship: Recombinants differ from parental allele combinations.

Representative question

Question 1

[Maximum number: 1]

An individual is heterozygous for two linked genes ABab\frac{\mathrm{AB}}{\overline{\mathrm{ab}}}.

To investigate the frequency of crossing over, a test cross is carried out between the individual and another that is homozygous recessive for both genes. What are the possible recombinants in the offspring of this cross?

A

Abab\frac{\mathrm{Ab}}{\mathrm{ab}} and Abab\frac{\mathrm{Ab}}{\mathrm{ab}}

B

ABab\frac{\mathrm{AB}}{\mathrm{ab}} and AbaB\frac{\mathrm{Ab}}{\mathrm{aB}}

C

Abab\frac{\mathrm{Ab}}{\mathrm{ab}} and aBab\frac{\mathrm{aB}}{\mathrm{ab}}

D

AAaa\frac{\mathrm{AA}}{\mathrm{aa}} and BBbb\frac{\mathrm{BB}}{\mathrm{bb}}

Retrieve the HL Inheritance Route

HL only

HL D3.2 is secure when chromosome behaviour explains the ratios: segregation and independent assortment produce unlinked dihybrid expectations, gene loci explain linkage, recombinants reveal crossing over, and chi-squared decides whether observed counts fit the expected model.

  • homologous chromosomes separate and random bivalent orientation assort unlinked genes
  • unlinked autosomal genes can produce 9:3:3:1 or 1:1:1:1 ratios
  • linked genes give more parental types and fewer recombinants after crossing over
  • observed counts are compared with expected ratios using df and p = 0.05

Solve HL Linkage and Chi-Squared Questions

HL only

HL inheritance transfer is about deciding whether the expected ratio should be Mendelian or linked, then testing the evidence. Start from meiosis and gene location, predict gametes or ratios, identify parental and recombinant classes, and use chi-squared when observed counts need a statistical conclusion.

  • Explain segregation and independent assortment from meiosis before using dihybrid ratios.
  • Use gene loci, linkage, crossing over, and recombinant frequency to interpret offspring classes.
  • Apply chi-squared with observed/expected values, degrees of freedom, p = 0.05, and a null-hypothesis conclusion.

Concept essentials

  • Recombinants differ from parental allele combinations.
  • Crossing over produces recombinant chromosomes during meiosis.
  • Recombination frequency can indicate distance between linked loci.