D2.3 Water potential
Water potential explains osmosis, solute effects, plant tissue changes, cell swelling, plasmolysis and isotonic medical conditions in living systems and cells.
- Syllabus
- First assessment 2025
- Topic
- D2.3
- Level
- HL
Water potential explains osmosis, solute effects, plant tissue changes, cell swelling, plasmolysis and isotonic medical conditions in living systems and cells.

Coverage 2016–2025 · Updated 16 Jul 2026
• Water forms hydration shells around ions and polar solutes
• Hydrogen bonding and charge attraction reduce free water movement
• Water moves by osmosis across partially permeable membranes
• It moves from hypotonic/lower solute solutions toward hypertonic/higher solute solutions
• Osmosis direction depends on internal and external solute concentration
• Isotonic conditions have dynamic water movement but no net osmosis
• Plant tissue changes mass or length when placed in sucrose solutions
• Percentage change graphs estimate isotonic/osmotic concentration
• Animal cells can lyse in hypotonic solutions and crenate in hypertonic solutions
• Freshwater protists use contractile vacuoles to expel excess water
• Plant cells become turgid in hypotonic solutions as vacuoles swell
• Hypertonic solutions cause flaccidity and plasmolysis from water loss
• Isotonic saline prevents harmful water gain or loss in body cells
• IV fluids and transplant organ baths must match tissue osmotic concentration
• Water potential is potential energy of water per unit volume, measured in kPa
• Pure water at standard conditions has water potential of 0 kPa
• Water moves from higher water potential to lower water potential
• Solutes lower water potential by restricting water molecule movement
• Water potential equals solute potential plus pressure potential
• Solute potential is zero or negative; pressure potential is often positive in walled cells
• Water entering plant cells increases pressure potential and dilutes solutes
• Water leaving plant cells lowers pressure potential and makes solute potential more negative