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D4.1 Natural selection

Natural selection explains how heritable variation, selection pressures, differential survival, reproduction and allele-frequency changes drive evolutionary adaptation in populations in evolving populations.

Syllabus
First assessment 2025
Topic
D4.1
Level
HL

Natural Selection Changes Populations through Differential Reproduction

Natural selection is the process in which heritable trait differences cause some individuals to leave more surviving offspring than others.

Selection pressure changes success; successful individuals pass alleles more often; across generations the population’s trait and allele frequencies shift.

Trace variation; pressure; survival or mating; offspring; population change.; separate variation, selection, inheritance and time

In a dry habitat, beetles whose colour better matches the soil are eaten less and leave more offspring, so camouflage becomes more common. This gives a concrete prediction from the stated population.

Individuals do not evolve because they need to; selection changes inherited variation already present. Interpret the result within the stated selection model and evidence limits.

Natural selection as mechanism

Assessment in practice

1–7 marks
How it is assessed

This objective is assessed through essay response, commonly using Explain / Identify / Evaluate.

Command terms

Explain / Identify / Evaluate / Outline / State / Describe / Discuss

What earns marks

Build the answer around this relationship: Natural selection acts on individuals but changes populations across generations.

Representative question

Question 1

[Maximum number: 8]

Explain how evolution may happen in response to environmental change with evidence from examples.

Mutation and Sex Create the Variation Selection Uses

Mutation introduces new DNA variants and sexual reproduction reshuffles existing alleles, supplying variation on which selection can act.

A mutation may alter a protein or regulation; meiosis and fertilization combine alleles in new ways. Selection then filters outcomes rather than producing the initial variation.

Label each source as new sequence change or allele reshuffling before predicting population variation.; separate variation, selection, inheritance and time

A mutation conferring insecticide resistance can spread faster when resistant and susceptible alleles are reshuffled into different offspring. This gives a concrete prediction from the stated population.

Most mutations are neutral or harmful; selection does not make every new variant advantageous. Interpret the result within the stated selection model and evidence limits.

Roles of mutation and sexual reproduction

Assessment in practice

1–7 marks
How it is assessed

This objective is assessed through structured response, commonly using Explain / Identify / Outline.

Command terms

Explain / Identify / Outline

What earns marks

Build the answer around this relationship: Mutation can create new alleles.

Representative question

Question 1

[Maximum number: 8]

Explain how sexual reproduction can eventually lead to evolution in offspring.

Overproduction Creates Competition for Limited Resources

When more offspring are produced than a habitat can support, individuals compete for food, space, mates or other limiting resources.

Resource limits turn small trait differences into differences in survival and reproduction. Competition is therefore a mechanism linking population size to selection pressure.

Name the limiting resource and the trait that changes access to it.; separate variation, selection, inheritance and time

A clutch produces ten hatchlings but the nest supports four; faster-feeding hatchlings obtain more food and are more likely to fledge. This gives a concrete prediction from the stated population.

Overproduction does not mean every species always exceeds carrying capacity; the model concerns potential versus available resources. Interpret the result within the stated selection model and evidence limits.

Overproduction and competition

Assessment in practice

1–3 marks
How it is assessed

This objective is assessed through structured response, commonly using Outline / Identify / State.

Command terms

Outline / Identify / State / Explain

What earns marks

Build the answer around this relationship: Overproduction means not all offspring survive to reproduce.

Representative question

Question 1

[Maximum number: 4]

Outline how overpopulation of a species in a given environment may lead to evolution.

Abiotic Conditions Can Select among Heritable Traits

Abiotic factors such as temperature, salinity, pH or drought act as selection pressures when they change survival or reproduction unequally.

The factor alters physiology or resource access; individuals with suitable heritable traits leave more offspring; allele frequencies can then change.

Link the environmental value to a measurable survival or reproductive consequence.; separate variation, selection, inheritance and time

During a drought, plants with deeper roots maintain water uptake and set more seeds than shallow-rooted plants. This gives a concrete prediction from the stated population.

A trait is not an adaptation merely because it is useful; inheritance and differential reproduction must be shown. Interpret the result within the stated selection model and evidence limits.

Abiotic factors as selection pressures

Assessment in practice

2 marks
How it is assessed

This objective is assessed through structured response, commonly using Outline / Explain.

Command terms

Outline / Explain

What earns marks

Build the answer around this relationship: Abiotic factors are non-living parts of the environment.

Representative question

Question 1

[Maximum number: 2]

Explain how natural selection is influenced by changes in the environment.

Fitness Means Relative Reproductive Success

Biological fitness is relative contribution of surviving offspring to the next generation, not simply strength, speed or lifespan.

A trait increases fitness only in a specified environment and comparison. Survival matters because it affects the number of offspring that inherit the trait.

Compare individuals in the same conditions using offspring contribution as the outcome measure.; separate variation, selection, inheritance and time

A smaller bird that produces eight fledglings can have higher fitness than a larger bird producing two. This gives a concrete prediction from the stated population.

Fitness is context-dependent; a trait advantageous in one habitat can be costly in another. Interpret the result within the stated selection model and evidence limits.

Differences in adaptation, survival, reproduction

Assessment in practice

1–2 marks
How it is assessed

This objective is assessed through multiple choice, commonly using Outline / Compare / Describe.

Command terms

Outline / Compare / Describe

What earns marks

Build the answer around this relationship: Adaptations are heritable traits that improve performance in context.

Representative question

Question 1

[Maximum number: 1]

In a natural population, what is a feature of individuals that are better adapted?

A

They start to produce offspring at a younger age than less well adapted individuals.

B

They produce identical offspring by cloning that are also better adapted.

C

They tend to produce more offspring during their lifetime than less well adapted individuals.

D

They do not produce more offspring than the environment can support.

Selection Requires Heritable Differences

For selection to produce an evolutionary change, the trait difference associated with reproductive success must be transmitted to offspring.

Environmental effects can change an individual’s phenotype without changing inherited alleles. Only the heritable component can shift population frequencies across generations.

Ask whether offspring resemble parents for the trait before attributing a long-term change to selection.; separate variation, selection, inheritance and time

If dark fur is inherited and dark mice leave more pups, dark alleles rise; if fur darkens only from soot exposure, the population need not evolve. This gives a concrete prediction from the stated population.

A trait can be heritable yet show little response when selection is weak or environments change. Interpret the result within the stated selection model and evidence limits.

Traits must be heritable

Assessment in practice

1 marks
How it is assessed

This objective is assessed through multiple choice, commonly using Identify.

Command terms

Identify

What earns marks

Build the answer around this relationship: Natural selection causes evolution only when selected traits are heritable.

Representative question

Question 1

[Maximum number: 1]

What is required for natural selection to occur?
I. Acquired characteristics
II. Advantageous characteristics
III. Genetic variation

A

I only

B

I and III only

C

II and III only

D

I, II and III

Sexual Selection Favors Mating Success

Sexual selection is selection for traits that increase access to mates or fertilization success, even when they carry survival costs.

Mate choice and competition among same-sex individuals change reproductive success. The trait spreads when its mating advantage outweighs its costs.

Separate survival benefit from mating benefit before explaining a conspicuous trait.; separate variation, selection, inheritance and time

Peacock tail feathers may attract mates while making escape harder, so mating success can favour the tail despite predation risk. This gives a concrete prediction from the stated population.

Sexual selection is one component of natural selection, not a guarantee that the trait improves survival. Interpret the result within the stated selection model and evidence limits.

Sexual selection

Assessment in practice

2–3 marks
How it is assessed

This objective is assessed through essay response, commonly using Explain / Suggest / Evaluate.

Command terms

Explain / Suggest / Evaluate

What earns marks

Build the answer around this relationship: Sexual selection acts through mating success.

Representative question

Question 1

[Maximum number: 6]

Explain what is meant by exaggerated traits and how they may develop in males of a species.

Selection Models Show Frequency Change over Generations

A selection model represents how different survival or reproduction rates alter genotype or allele frequencies through repeated generations.

Set initial frequencies and fitnesses; calculate surviving contributions; normalize the next generation; repeat. The direction depends on assumptions and starting values.

Check which class has the highest reproductive contribution before reading a model’s trend.; separate variation, selection, inheritance and time

If resistant bacteria leave twice as many offspring as susceptible bacteria under antibiotic exposure, the resistant frequency rises over successive cycles. This gives a concrete prediction from the stated population.

A model is not a forecast without its assumptions; migration, mutation and changing pressures can alter the trend. Interpret the result within the stated selection model and evidence limits.

Modelling selection

Assessment in practice

1–4 marks
How it is assessed

This objective is assessed through multiple choice, commonly using Describe / Explain.

Command terms

Describe / Explain

What earns marks

Build the answer around this relationship: Models can show how selection changes variant frequencies over generations.

Representative question

Question 1

[Maximum number: 1]

John Endler experimented on populations of guppies (Poecilia reticulata) with different colouration. A male guppy fish is shown with large spots, which makes the fish more attractive to females, but more visible to predators.

The table shows the male colouration of guppy fish and number of predators in three different ponds.

\begin{tabular}{|l|l|l|l|}
\hline & Predator X & Predator Y & Male guppy colouration \\
\hline Pond 1 & 12 & 0 & Large colourful spots \\
\hline Pond 2 & 15 & 0 & Medium colourful spots \\
\hline Pond 3 & 5 & 17 & None/very small drab spots \\
\hline
\end{tabular}

What can be concluded from the data?

A

There is a positive correlation between numbers of predator X and size of spots.

B

Predator Y has least influence on colouration.

C

There is a negative correlation between number of predators and size of spots.

D

There is no sexual selection.

Retrieve the Core Natural Selection Route

Core D4.1 examples follow the same causal route: heritable variation exists, a selection pressure acts, individuals differ in fitness, and alleles linked to higher reproduction become more common. Endler’s guppies and sexual selection are evidence versions of the same chain.

  • mutation creates alleles; meiosis and fertilization reshuffle combinations
  • overproduction, limited resources, and abiotic factors filter variants
  • passing alleles to offspring in a particular environment
  • Endler controlled predation pressure and guppy colour patterns changed

Core Natural Selection

Core natural-selection exam answers should never stop at “the best adapted survive.” They need the chain: heritable variation exists, a named pressure acts, some individuals have higher fitness, and their alleles become more common over generations. Use this for abiotic pressure, overproduction, sexual selection, and Endler-style data.

  • Explain natural selection using heritable variation, selection pressure, differential survival/reproduction, and population change.
  • Distinguish mutation/recombination as sources of variation from selection as the filtering process.
  • Use examples such as abiotic pressure, sexual selection, or Endler guppy data to support the chain.

A Gene Pool Contains Population Alleles

HL only

A gene pool is the collection of all alleles present in an interbreeding population at a given time.

Counting alleles across individuals lets us describe population genetic composition and compare it across generations. It is a population property, not one organism’s genome.

Define the population boundary and count allele copies before comparing gene pools.; separate variation, selection, inheritance and time

Ten diploid individuals carry 20 copies at a locus; those copies make the population’s gene pool for that locus. This gives a concrete prediction from the stated population.

A gene pool is not the sum of every species in an ecosystem; it is tied to a breeding population. Interpret the result within the stated selection model and evidence limits.

Gene pool concept

HL only

Assessment in practice

1–3 marks
How it is assessed

This objective is assessed through structured response, commonly using State / Discuss.

Command terms

State / Discuss

What earns marks

Build the answer around this relationship: A gene pool includes all alleles in a population.

Representative question

Question 1

[Maximum number: 3]

Discuss how the isolation of populations due to the fragmentation of forests could lead to changes in gene pools.

Allele Frequency Is a Proportion of Gene Copies

HL only

Allele frequency is the proportion of copies of an allele among all copies at a locus in a population.

In a diploid sample, count each allele copy, divide by total copies, and compare the proportion through time. Genotype frequency and allele frequency are related but different.

Count copies rather than individuals carrying the allele, then divide by the total allele count.; separate variation, selection, inheritance and time

If 30 of 100 allele copies are A, the frequency of A is 0.30, not the fraction of individuals with at least one A. This gives a concrete prediction from the stated population.

The denominator changes with ploidy and sampling; state the population and locus. Interpret the result within the stated selection model and evidence limits.

Allele frequencies

HL only

Assessment in practice

1 marks
How it is assessed

This objective is assessed through structured response, commonly using State / Deduce.

Command terms

State / Deduce

What earns marks

Build the answer around this relationship: Allele frequency is a proportion within the population gene pool.

Representative question

Question 1

[Maximum number: 1]

Define allele frequency.

Selection, Drift, Migration and Mutation Can Change Frequencies

HL only

Allele frequencies change when selection, genetic drift, gene flow or mutation changes which alleles are represented in the next generation.

Selection is non-random reproductive difference; drift is sampling chance; migration moves alleles; mutation creates new variants. Their relative strengths determine the pattern.

Identify the process and its expected direction before interpreting a frequency change.; separate variation, selection, inheritance and time

A storm randomly leaves mostly one colour of beetle (drift), whereas a predator consistently removes the visible colour (selection). This gives a concrete prediction from the stated population.

A frequency change alone cannot identify the cause without population and environmental evidence. Interpret the result within the stated selection model and evidence limits.

Changes in allele frequency

HL only

Assessment in practice

1–3 marks
How it is assessed

This objective is assessed through essay response, commonly using Compare / Outline.

Command terms

Compare / Outline

What earns marks

Build the answer around this relationship: Selection changes allele frequencies through differential reproductive success.

Representative question

Question 1

[Maximum number: 4]

If a wild population of cats contained both curled ears and normal ears, explain how the proportion of these two phenotypes could change in the population.

Different Selection Patterns Shape Trait Distributions

HL only

Directional selection shifts a trait mean; stabilizing selection favours intermediate values; disruptive selection favours extremes.

The pattern depends on which phenotypes have higher fitness. Repeated selection changes the distribution, not merely one individual’s trait.

Compare fitness at low, intermediate and high trait values before naming the pattern.; separate variation, selection, inheritance and time

If both very small and very large beaks feed better than medium beaks, disruptive selection can widen the distribution. This gives a concrete prediction from the stated population.

The labels describe the observed fitness pattern; they do not specify the genes or guarantee speciation. Interpret the result within the stated selection model and evidence limits.

Types of selection

HL only

Assessment in practice

1 marks
How it is assessed

This objective is assessed through structured response, commonly using Explain / Identify.

Command terms

Explain / Identify

What earns marks

Build the answer around this relationship: Directional selection shifts the population toward one extreme.

Representative question

Question 1

[Maximum number: 2]

Phenotypic variation allows natural selection within populations. Compare and contrast directional and disruptive selection.

Hardy–Weinberg Relates Allele and Genotype Frequencies

HL only

For two alleles with frequencies p and q, the Hardy–Weinberg model predicts p², 2pq and q² genotype frequencies under specified assumptions.

The equation p+q=1 describes allele frequencies; p²+2pq+q²=1 follows random union of gametes. It provides a null expectation for detecting evolutionary forces.

State p and q; calculate expected genotypes; compare observed values with the model.; separate variation, selection, inheritance and time

If q=0.2, expected aa frequency is q²=0.04 and heterozygote frequency is 2pq=0.32. This gives a concrete prediction from the stated population.

The model is an expectation, not a claim that real populations never evolve. Interpret the result within the stated selection model and evidence limits.

Hardy-Weinberg equation

HL only

Assessment in practice

2–3 marks
How it is assessed

This objective is assessed through essay response, commonly using Outline / Discuss.

Command terms

Outline / Discuss

What earns marks

Build the answer around this relationship: p and q are allele frequencies that add to one.

Representative question

Question 1

[Maximum number: 3]

Discuss the use of the Hardy-Weinberg equation in population genetics studies.

Hardy–Weinberg Needs Restrictive Conditions

HL only

Hardy–Weinberg equilibrium assumes a large population, random mating, no migration, no mutation and no selection.

These conditions prevent systematic changes in allele frequencies or sampling noise. Violating one condition can make observed genotype frequencies depart from expectation.

Check each assumption before using an equilibrium calculation as a biological conclusion.; separate variation, selection, inheritance and time

A small isolated population can deviate because drift acts strongly even if no selection is present. This gives a concrete prediction from the stated population.

A departure does not reveal which assumption failed; independent evidence is needed. Interpret the result within the stated selection model and evidence limits.

Hardy-Weinberg conditions

HL only

Assessment in practice

1–2 marks
How it is assessed

This objective is assessed through structured response, commonly using State / Identify.

Command terms

State / Identify

What earns marks

Build the answer around this relationship: Hardy-Weinberg equilibrium is a null model for no evolution.

Representative question

Question 1

[Maximum number: 2]

State two assumptions made when using the Hardy-Weinberg equation.
1.

Artificial Selection Directs Breeding Choices

HL only

Artificial selection occurs when humans choose which organisms reproduce to increase a desired inherited trait.

Repeated selection raises the frequency of alleles associated with the chosen phenotype, often faster than natural environments would. Trade-offs can reduce genetic diversity or expose harmful linked traits.

Identify the human criterion, breeding decisions and inherited response across generations.; separate variation, selection, inheritance and time

Breeders repeatedly choose dairy cattle with high milk yield, increasing average yield but potentially narrowing the genetic base. This gives a concrete prediction from the stated population.

Artificial selection is not genetic engineering; it changes reproduction choices rather than directly editing DNA. Interpret the result within the stated selection model and evidence limits.

Retrieve the HL Population Genetics Route

HL only

HL D4.1 turns selection into measurable population genetics. A gene pool changes when allele frequencies shift. Hardy-Weinberg gives a no-evolution baseline; selection graphs, isolated populations, artificial selection, and resistance show how forces move populations away from that baseline.

  • all alleles in an interbreeding population
  • directional, stabilizing, or disruptive selection favours different phenotype ranges
  • p and q calculate allele and genotype frequencies in equilibrium
  • selection, mutation, migration, drift, or non-random mating may be acting

HL Population Genetics

HL only

HL population-genetics questions ask students to quantify or model evolution. The answer starts with the gene pool and allele frequencies, then uses the model or selection graph to decide whether the population is at equilibrium or being shifted by selection, mutation, migration, drift, artificial selection, or isolation.

  • Use gene pool and allele frequency language to define evolution quantitatively.
  • Interpret selection graphs and isolated populations as changes in phenotype or allele frequencies.
  • Apply Hardy-Weinberg equations and equilibrium assumptions, then explain what deviations mean.
ConceptIB Biology HL