C2.2.2—Resting potential generation
Resting potential is generated when ion pumps and membrane permeability make the inside of an axon negative relative to the outside.
- Syllabus
- First assessment 2025
- Objective
- C2.2.2
- Level
- HL
Resting potential is generated when ion pumps and membrane permeability make the inside of an axon negative relative to the outside.

Coverage 2018–2023 · Updated 15 Jul 2026
A resting neuron has a stable negative membrane potential created by ion gradients and selective membrane permeability.
The sodium–potassium pump maintains high K⁺ inside and Na⁺ outside. Leak channels let more K⁺ leave than Na⁺ enter, leaving the inside relatively negative until electrical and chemical forces balance. At rest, selective ion permeability and active transport keep the membrane ready to respond to a new stimulus.
Explain resting potential with:
If K⁺ leak channels close, the membrane potential shifts because the main outward current has changed.
The pump maintains gradients over time; it does not produce each action potential directly.
This objective is assessed through structured response, commonly using Define / Outline / Explain.
Define / Outline / Explain / State / Label
Build the answer around this relationship: The resting axon is negative inside relative to outside.
Representative question
Outline how neurons generate a resting potential.
a. sodium-potassium pump
b. sodium /Na+out and potassium /K+in
OR
sodium /Na+concentration higher outside and potassium /K+higher inside
c. three Na+pumped for every two K+(hence negative inside)
OR
inside of axon holds negative ions/ Cl−ions/negatively charged proteins/organic anions (hence negative inside)
d. by active transport / using ATP
e. inside (of axon/neuron) is negative in comparison to outside
OR
electrochemical concentration/charge difference (across the membrane) is the resting potential
f. resting potential is -70 mV
4 max
Neurons carry electrical impulses in the nervous system; motor, sensory, and relay neurons differ in axon, dendrite, and cell body arrangement. Sodium-potassium pumps use ATP to move 3 Na+ out and 2 K+ in; ion gradients make the resting axon polarized at about -70 mV. A nerve impulse is a propagated action potential along a nerve fibre; stimulus-triggered sodium influx reverses membrane polarity. Larger axon diameter lowers resistance and increases impulse speed; myelin sheaths and nodes of Ranvier enable faster saltatory conduction. Synapses connect neurons to neurons, muscles, or glands; chemical synapses transmit one way across a narrow synaptic cleft. Action potentials open voltage-gated Ca2+ channels in presynaptic terminals; Ca2+ causes vesicle fusion and neurotransmitter exocytosis into the cleft. Neurotransmitters diffuse and bind receptors on the postsynaptic membrane; EPSPs depolarize the membrane and make threshold more likely.