Course review

C1.3 Photosynthesis

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Learning objective

C1.3.1—Light energy → chemical energy

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• Photosynthesis converts light energy into chemical energy in carbon compounds • Photoautotrophs use chlorophyll in chloroplasts or cyanobacterial membranes

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Learning objective

C1.3.2—CO₂ → glucose

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• Carbon dioxide is reduced to carbohydrate using hydrogen from water • Glucose represents the main stored product, though many compounds are synthesized

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Learning objective

C1.3.3—Oxygen as by-product

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• Oxygen is released from photolysis of water, not directly from CO₂ • Oxygenic photosynthesis occurs in plants, algae, and cyanobacteria

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Learning objective

C1.3.4—Photosynthetic pigment separation

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• Paper chromatography separates chlorophylls and accessory pigments • Rf values compare pigment movement relative to solvent front

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Learning objective

C1.3.5—Absorption of specific wavelengths

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• Chlorophyll and accessory pigments absorb specific wavelengths • Absorbed light excites electrons for light-dependent reactions

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Learning objective

C1.3.6—Absorption vs. action spectra

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• Absorption spectra show wavelengths absorbed by pigments • Action spectra show wavelengths most effective for photosynthesis rate

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Learning objective

C1.3.7—Limiting factors investigation

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• Light intensity, CO₂ concentration, and temperature can limit photosynthesis • Investigations change one variable and estimate rate from O₂ production or CO₂ uptake

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Learning objective

C1.3.8—CO₂ enrichment experiments

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• CO₂ enrichment tests whether increased CO₂ raises photosynthesis or growth • Greenhouse and FACE experiments compare realistic crop and ecosystem responses

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Learning objective

C1.3.9 (HL)—Photosystems

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• Photosystems are pigment-protein arrays in thylakoid membranes • Antenna pigments pass energy to reaction-centre chlorophyll

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Learning objective

C1.3.10 (HL)—Advantages of pigment arrays

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• Pigment arrays broaden wavelength absorption and funnel energy efficiently • A single chlorophyll molecule cannot sustain the full light reaction system

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Learning objective

C1.3.11 (HL)—Photolysis of water

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• Photosystem II uses light energy to split water • Photolysis supplies replacement electrons, protons, and oxygen

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Learning objective

C1.3.12 (HL)—ATP production in thylakoids

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• Electron transport pumps protons into the thylakoid space • Proton flow through ATP synthase produces ATP by photophosphorylation

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Learning objective

C1.3.13 (HL)—NADP reduction

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• Photosystem I re-excites electrons for NADP reduction • NADP accepts electrons and H⁺ to form reduced NADP/NADPH

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Learning objective

C1.3.14 (HL)—Thylakoids as light-dependent systems

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• Thylakoids organize photosystems, electron carriers, and ATP synthase • Key outputs on the stromal side are ATP and reduced NADP

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Learning objective

C1.3.15 (HL)—Carbon fixation by Rubisco

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• Rubisco fixes CO₂ to RuBP, forming glycerate 3-phosphate • Rubisco is abundant but slow and limited by low CO₂

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Learning objective

C1.3.16 (HL)—Triose phosphate synthesis

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• Glycerate 3-phosphate is reduced to triose phosphate • ATP supplies energy and reduced NADP supplies hydrogen

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Learning objective

C1.3.17 (HL)—RuBP regeneration

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• Most triose phosphate regenerates RuBP so the Calvin cycle continues • ATP is required for regeneration of the CO₂ acceptor

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Learning objective

C1.3.18 (HL)—Synthesis of other compounds

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• Triose phosphate is converted into sugars, starch, lipids, and organic acids • Mineral nutrients allow synthesis of amino acids and other compounds

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Learning objective

C1.3.19 (HL)—Interdependence of light and light-independent reactions

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• Light-dependent reactions supply ATP and reduced NADP for the Calvin cycle • The Calvin cycle returns ADP and NADP and depends on CO₂/hydrogen carbonate availability

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