IB Biology HL C1.3 Photosynthesis Questions
Practise IB Biology HL C1.3 by linking pigments and limiting factors to photosystems, photophosphorylation and carbon fixation evidence.
- Syllabus
- First assessment 2025
- Course
- Biology HL
- Level
- HL
Practise IB Biology HL C1.3 by linking pigments and limiting factors to photosystems, photophosphorylation and carbon fixation evidence.
In ecosystems, energy is used to convert inorganic compounds into organic matter. Energy enters ecosystems through producers.
Explain the processes by which light energy is converted into chemical energy.
a. plants/producers/autotrophs convert light to chemical energy by photosynthesis
b. chlorophyll/photosynthetic pigments absorb light
c. electrons are excited/raised to higher energy level
d. excited electrons pass along chain of electron carriers
e. energy from electrons used to pump protons across thylakoid membrane/into thylakoid space
f. chemiosmosis/proton gradient used to make ATP
g. ATP synthase generates ATP
h. pigments arranged in photosystems
i. electrons from Photosystem II flow via the electron chain to Photosystem I
j. electrons from Photosystem I are used to reduce NADP
k. ATP and reduced NADP used in the light independent reactions/Calvin cycle
I. carbohydrate/glucose/carbon compounds produced containing energy
In water, shorter wavelengths of light such as blue and green penetrate deepest, and longer wavelengths of light penetrate less far. Red light does not reach depths of more than 50 m . Marine algae live at various water depths. Certain species of algae contain the pigments that enable them to adapt to life at different depths.
The graphs show the absorption and action spectra for two species of algae: Ulva taeniata, which is green, and Porphyra nereocystis, which is red.
Absorption / \%
In each species, identify the colour of light that is used mainly for photosynthesis.
Ulva taeniata:
Porphyra nereocystis:
Ulva taeniata: violet/blue;
Porphyra nereocystis: green OR blue/green;
For Ulva, accept either violet or blue.
For Porphyra, do not accept only blue.
Deduce, with a reason, which algae lives at a greater depth.
a. P. nereocystis because action spectrum/photosynthesis is very low in red light/longer wavelength
OR
P. nereocystis because action spectrum/photosynthesis is higher in green/blue light (which can penetrate deeper waters)
OR
P. nereocystis as it is not dependant on red light for photosynthesis but uses green/blue light;
b. (theoretically) U. taeniata as it has the highest action spectrum at the shortest wavelengths/violet/blue;
Marking guidance:
Do not accept answers referring to the absorption/reflection only.
Accept answers referring to wavelength instead of colour.
1
max
Describe how the rate of photosynthesis could be measured in a marine alga, to produce an action spectrum.
a. expose algae to different light wavelengths/colour/filters;
b. at a fixed distance/intensity;
c. (measure) levels of oxygen/carbon dioxide/pH;
d. per/over (a fixed period of) time;
e. other named controlled variable: temperature/mass of seaweed/volume of water/salt concentration;
f. plot wavelength ( x -axis) against the named/dependent variable ( y -axis);
[Max 2] if different wavelengths/colours/filters are not mentioned.
c. and d. Accept oxygen produced per time, also in a formula or 'rate of oxygen production' for 2 marks.
c. Do not accept bubbles only.
e. Only 1 variable required. Do not accept controlled environment.
Marking guidance:
Do not accept rate of photosynthesis as it's in the stem.
3
max
Outline the structure and location of photosystems.
Location:
a. located in thylakoid (membranes);
b. photosystem/PS II is linked to Photosystem/PS I (by electron carriers)
OR
PS II is located before PS I OWTTE;
Structure:
c. reaction center/chlorophyll/primary pigment AND accessory pigments / light harvesting complex/antenna;
d. P680/P700/special chlorophyll (a) as the reaction centre;
b. Do not accept only the names of the photosystems without reference to linkage or order.
c. Accept 'various' or 'an array of' pigments for accessory pigments.
2
Marking guidance:
max
State the method used to separate photosynthetic pigments in a leaf extract.
(thin layer/paper) chromatography;
Distinguish between the absorption of red light, blue light and green light by plants.
a. chlorophyll is the main photosynthetic pigment;
b. high levels of absorption in red light and blue light;
(both needed)
c. greatest absorption in blue light;
d. least/low absorption in green light;
e. green light is reflected;
f. other pigments absorb other wavelengths/colours;
Outline how light intensity and concentration of carbon dioxide affect photosynthesis.
a. low light intensity affects light-dependent reactions;
b. fewer electrons are excited / less photolysis occurs;
c. less NADPH and ATP produced at low light intensities;
(both needed)
d. rate-limiting step is the reduction of G3P/glycerate 3-phosphate/PGA phosphoglycerate;
e. graph showing: effect of light intensity on rate of photosynthesis;
(must not start at zero)
f. low carbon dioxide concentration affects the Calvin cycle/light-independent stage;
g. fixation of CO2 is decreased;
h. less ribulose bisphosphate joins to CO2 to form G3P/glycerate 3-phosphate/PGA phosphoglycerate;
i. graph showing: effect of CO2 concentration on rate of photosynthesis;