CAIE IGCSE Biology 6.1 Photosynthesis Questions

Practise defining photosynthesis, writing its equations and analysing investigations involving light, chlorophyll, carbon dioxide, oxygen production and limiting factors.

Syllabus
2026–2028
Course
Biology 0610

Exam points

  • Define photosynthesis as using light energy to make carbohydrates from carbon dioxide and water, and write the word or balanced chemical equation with correct products.
  • Explain the roles of chloroplasts and chlorophyll in absorbing and transferring light energy into chemical energy used to synthesise carbohydrates.
  • Explain how photosynthetic glucose is converted to starch, sucrose or cellulose, and how nitrate and magnesium ions support plant growth and chlorophyll production.
  • Interpret starch tests, gas-exchange experiments and light/dark indicator data to identify where photosynthesis occurs and how it relates to respiration.
  • Calculate or interpret photosynthesis rate and identify limiting factors such as light intensity, carbon dioxide concentration, temperature, water supply or humidity.

Question 1

[Maximum number: 1]

Which substance is used up in photosynthesis?

A

chlorophyll

B

light

C

oxygen

D

water

Question 2

[Maximum number: 1]

The following can be used to write a word equation for photosynthesis.

1 carbon dioxide and water

2 light and chlorophyll
3 glucose and oxygen

Which shows a correct word equation for photosynthesis?

A

1→21 \rightarrow 2 in the presence of 3

B

1→31 \rightarrow 3 in the presence of 2

C

2→32 \rightarrow 3 in the presence of 1

D

3→13 \rightarrow 1 in the presence of 2

Question 3

[Maximum number: 1]

Which structure contains the pigment that transfers energy from light into chemical energy?

A

chloroplast

B

mitochondrion

C

nucleus

D

ribosome

Question 4

[Maximum number: 2]

Fig. 4.1 is a photograph of a water lily, Nymphaea alba, in a lake.

Fig. 4.1

Fig. 4.1

Fig. 4.2 is a photomicrograph of a cross-section of part of a water lily leaf.

Fig. 4.2 is a photomicrograph of a cross-section of part of a water lily leaf.

A green pigment is found in tissue B in Fig. 4.2.

Describe the function of this pigment.

Question 5

[Maximum number: 2]

State the balanced chemical equation for photosynthesis.

Question 6

[Maximum number: 3]

Scientists investigated the effect of cuticle thickness on water loss from the leaves of the balsam fir tree, Abies balsamea.

The leaves were divided into three groups:

A - thick cuticle

B - medium cuticle

C - thin cuticle

Samples of leaves from each group were weighed. The leaves were placed on a tray in dry air at 20∘C20^{\circ} \mathrm{C}. The samples of leaves were reweighed, at intervals, over 15 hours. The scientists calculated the mass of each sample of leaves as a percentage of the initial mass.

mass of the leaves as a percentage of the initial mass

Fig. 5.1 shows the results.

Fig. 5.1 shows the results.

Water is one of the raw materials needed for the production of sugars in photosynthesis.

State three ways a plant uses the sugars produced in photosynthesis.

Question 7

[Maximum number: 1]

Some algae are single-celled organisms that can photosynthesise. Their cells contain the pigment chlorophyll.

State the name of one mineral ion that is needed to make chlorophyll.

Question 8

[Maximum number: 1]

A plant with variegated leaves was placed in a dark cupboard for 48 hours so that all the starch was removed from the leaves. The variegated leaves have green parts and white parts.

Black paper was then fixed on one leaf and the plant was exposed to the light.
After 24 hours, which region of the leaf contained starch?

Figure for Question 8 — CAIE IGCSE Biology

Question 9

[Maximum number: 1]

The diagram shows an experiment investigating the effect of light intensity on an aquatic plant.

Figure for Question 9 — CAIE IGCSE Biology
Figure for Question 9 — CAIE IGCSE Biology

Photosynthesis occurred in both test-tube P and test-tube Q . Both test-tubes were kept at the same temperature. The number of bubbles produced in test-tube P was 12 bubbles per minute.

What is the most likely number of bubbles produced in one minute in test-tube Q ?

A

0

B

3

C

12

D

48

Question 10

[Maximum number: 4]

A student investigated the effect of light on photosynthesis in algae, using hydrogencarbonate indicator solution.

Carbon dioxide is an acidic gas.

Table 4.1 shows the colour of the hydrogencarbonate indicator solution at different pH values.

Table 4.1

Table 4.1

Two test-tubes were set up at the same time. Both contained algae and hydrogencarbonate indicator. At the start of the investigation the hydrogencarbonate indicator was red in both test-tubes.

One test-tube was placed in the dark and one test-tube was placed in the light.
After 20 minutes, the contents of the test-tube in the dark were yellow and the contents of the test-tube in the light were purple.

Explain these results.

Question 11

[Maximum number: 3]

A student investigated the effect of light on photosynthesis in algae, using hydrogencarbonate indicator solution.

Carbon dioxide is an acidic gas.

Table 4.1 shows the colour of the hydrogencarbonate indicator solution at different pH values.

Table 4.1

Table 4.1

Fig. 4.1 is a graph showing the effect of light intensity on the rate of photosynthesis.

Fig. 4.1

Fig. 4.1

State which factors could be limiting the rate of photosynthesis at X and at Y in Fig. 4.1.

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