CAIE IGCSE Biology 6 Plant Nutrition
Practise photosynthesis equations, leaf adaptations, limiting factors and experimental evidence for plant carbohydrate production.
- Syllabus
- 2026–2028
- Course
- Biology 0610
Practise photosynthesis equations, leaf adaptations, limiting factors and experimental evidence for plant carbohydrate production.
Which substance is used up in photosynthesis?
chlorophyll
light
oxygen
water
D
Scientists investigated the effect of cuticle thickness on water loss from the leaves of the balsam fir tree, Abies balsamea.
The leaves were divided into three groups:
A - thick cuticle
B - medium cuticle
C - thin cuticle Samples of leaves from each group were weighed. The leaves were placed on a tray in dry air at 20∘C. The samples of leaves were reweighed, at intervals, over 15 hours. The scientists calculated the mass of each sample of leaves as a percentage of the initial mass. mass of the leaves as a percentage of the initial mass

Fig. 5.1 shows the results.
Water is one of the raw materials needed for the production of sugars in photosynthesis.
State the name of the other raw material needed for photosynthesis.
carbon dioxide ;
State three ways a plant uses the sugars produced in photosynthesis.
respiration ;
starch ;
cellulose ;
chlorophyll ;
sucrose ;
nectar ;
amino acids / protein ;
fats / oils ;
nucleic acids / DNA / RNA ;
growth of (any named part) membrane, cell wall, cytoplasm ;
Fig. 4.1 is a photomicrograph of a cross section of part of a leaf.

Fig. 4.1
Identify the tissue labelled A in Fig. 4.1.
A - (upper) epidermis ;
R lower epidermis
Identify the structure labelled C in Fig. 4.1.
C - vascular bundle ;
A xylem and phloem / vein
Describe how the tissue labelled B is adapted to maximise photosynthesis.
B is the palisade, mesophyll / tissue / layer / cells ;
cells are tightly packed / AW ; ref. to many chloroplasts / lots of chlorophyll ;
(cells) positioned at the top of the leaf ;
(large vacuole) ensures chloroplasts are at the edge of cells ;
AVP ;
The effect of carbon dioxide concentration on the rate of oxygen production in an aquatic plant was measured.
- A lamp was used to keep the light intensity constant.
- The oxygen gas released by the plant was collected in a gas syringe.
- The plant was placed in water that was kept constant at 20∘C.
Fig. 4.2 shows the results.
The rate of oxygen production was assumed to be the same as the rate of photosynthesis.
Suggest why the rate of oxygen production was not the same as the rate of photosynthesis.

Fig. 4.2
not all the oxygen is, released / collected / measured (from the plant) ; some is used in respiration ; some is dissolved in the water ;
(therefore) the rate of photosynthesis may appear lower than it actually is ;
Explain the results shown in Fig. 4.2.
1 carbon dioxide is required for photosynthesis ;
2 initially carbon dioxide concentration is limiting the rate of photosynthesis ;
3 (after 500μ mol per dm3 ) carbon dioxide concentration is not limiting the rate of photosynthesis / other factors are limiting the rate of photosynthesis ;
4 ref. to named limiting factor ;
The investigation was repeated with the same type of aquatic plant at 10∘C.
Draw a line on Fig. 4.2 to predict the results at 10∘C.
initial gradient is less steep ; plateau below original line ;
Which structure contains the pigment that transfers energy from light into chemical energy?
chloroplast
mitochondrion
nucleus
ribosome
A