CAIE IGCSE Biology 6.1.9 Aquatic Plant Gas Exchange in Light & Dark
Practise using hydrogencarbonate indicator to compare net carbon dioxide exchange by aquatic plants in light and darkness.
- Syllabus
- 2026–2028
- Course
- Biology 0610
Practise using hydrogencarbonate indicator to compare net carbon dioxide exchange by aquatic plants in light and darkness.
A student investigated the effect of light on photosynthesis in algae, using hydrogencarbonate indicator solution.
Carbon dioxide is an acidic gas.
Table 4.1 shows the colour of the hydrogencarbonate indicator solution at different pH values.

Table 4.1
Two test-tubes were set up at the same time. Both contained algae and hydrogencarbonate indicator. At the start of the investigation the hydrogencarbonate indicator was red in both test-tubes.
One test-tube was placed in the dark and one test-tube was placed in the light.
After 20 minutes, the contents of the test-tube in the dark were yellow and the contents of the test-tube in the light were purple.
Explain these results.
any four from:
1 (algae) photosynthesise in light / no photosynthesis in the dark ;
2 carbon dioxide is, used / absorbed / taken / AW, in photosynthesis ;
3 (purple because CO2 used in) photosynthesis results in, pH increase / becomes (more) alkaline / less acidic ;
4 (algae) respire in light and dark ;
5 carbon dioxide is, produced / released / AW, in respiration ;
6 in light there is a higher (rate of) photosynthesis than (the rate of) respiration ;
7 (yellow because CO2 given off from) respiration results in, pH decrease / more acidic / less alkaline / becomes (nearer to) neutral ;