CAIE IGCSE Biology 20.4.8 AI & IVF in Captive Breeding
Practise sequencing artificial insemination and IVF and comparing where gametes meet and embryos develop or are transferred.
- Syllabus
- 2026–2028
- Course
- Biology 0610
Practise sequencing artificial insemination and IVF and comparing where gametes meet and embryos develop or are transferred.
Fig. 6.1 shows a boreal toad, Anaxyrus boreas. This toad is listed as an endangered species.

Fig. 6.1
There are conservation programmes to reintroduce boreal toads into their natural habitat.
Conservation programmes for other endangered animals, such as birds, use artificial insemination as part of a captive breeding programme.
Describe how artificial insemination is carried out as part of a captive breeding programme.
any four from: collect, sperm / gamete / semen (from male) ; males can be chosen from different breeding programmes ; to maximise genetic variation ; sperm, screened / quality checked / AW ; sperm / semen, frozen ; (female) given drugs to induce ovulation ; sperm / semen, placed in, vagina / uterus / oviduct ; sperm / semen, inserted at appropriate time for ovulation ;
A cloaca