A sigmoid growth model can be tested by repeatedly measuring a population grown under controlled conditions, such as yeast or duckweed.
Start replicate cultures with comparable initial populations, keep temperature, nutrients, volume and light conditions controlled, measure population size at regular intervals, calculate means, and plot population size against time.
Look for an initially increasing growth rate, a transition as limiting factors intensify, and a plateau near carrying capacity. Compare replicate data with the ideal S-shaped curve and identify deviations.
Duckweed frond number can be counted daily in replicate containers; nutrient depletion, shading and crowding eventually reduce net growth and produce a plateau.
The sigmoid is a model. A plateau may shift if conditions change, and deviations must not be hidden by forcing a fitted curve through poor data.