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CAIE A-Level Biology 19.1 Genetic Technology Principles Question Bank

Practise genetic-technology principle questions by tracing recombinant DNA steps, PCR amplification, gel electrophoresis, gene editing and molecular databases.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • trace recombinant DNA work using gene sources, restriction enzymes, ligase, plasmids and promoters
  • explain PCR components, cycles and Taq polymerase before gel or sequencing analysis
  • interpret marker genes, gene editing, microarrays or sequence databases in applied contexts

Covered syllabus points

19.1.1—Recombinant DNA definition19.1.2—Genetic engineering and gene expression19.1.3—Sources of genes19.1.4—Enzymes and vectors in gene transfer19.1.5—Promoter transfer alongside desired gene19.1.6—Marker genes for gene expression19.1.7—Gene editing by insertion, deletion or replacement19.1.8—PCR to clone and amplify DNA19.1.9—Gel electrophoresis19.1.10—Microarrays in genome analysis19.1.11—Sequence and protein databases

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