Practise explaining why transferred genes need promoters, how promoter choice controls expression and how plasmid orientation determines whether a gene is transcribed.
Factor VIII can be made as a recombinant human protein.
The gene that codes for human factor VIII can be transferred into mammalian cells in tissue culture.
Explain why a promoter also needs to be transferred into the mammalian cells so that human factor VIII can be synthesised.
any two from:
1 required, for gene expression / to start transcription ;
2 (so) transcription factor can bind (to promoter)-;
3 so RNA polymerase can bind (to promoter) ;