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CAIE A-Level Biology A2 19.1.6 Marker Genes for Gene Expression Questions

Practise placing fluorescent markers beside a target gene, detecting GFP expression and interpreting fluorescence or its loss as evidence of transfer, expression or deletion.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • Place a fluorescent marker gene with or beside the gene of interest, using linked transfer or the same promoter so marker expression indicates successful transfer or expression of the target gene.
  • Detect the fluorescent marker product under UV or blue light to identify transformed cells, tissues or organisms and confirm where the transferred gene is expressed.
  • Interpret presence or loss of fluorescence as evidence that the marker gene remains expressed or has been deleted by a gene-editing treatment.

CAIE A-Level Biology A2 19.1.6 Marker Genes for Gene Expression Questions question 1

[Maximum number: 2]

Traditional techniques for genetically modifying organisms use three enzymes:
- restriction endonuclease
- reverse transcriptase
- DNA ligase.

For example, these enzymes have been used to produce genetically modified (transgenic) pigs containing the GFP gene coding for green fluorescent protein, originally sourced from jellyfish.

Explain why the GFP gene was chosen for testing the new technique.

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