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CAIE A-Level Biology 19.1.4 Enzymes and Vectors in Gene Transfer

Practise assigning restriction endonuclease, ligase, polymerase and reverse transcriptase to recombinant-DNA stages and evaluating plasmid vectors.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • match restriction endonuclease, ligase and polymerases to each recombinant-DNA step
  • explain sticky-end annealing and ligase formation of phosphodiester bonds in plasmids
  • evaluate plasmid restriction sites, markers, replication and stability as vector features

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