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CAIE A-Level Biology 19.1.8 PCR and DNA Amplification

Practise sequencing PCR temperatures and molecular events, explaining reagent roles and using cycle or threshold data to quantify DNA amplification.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • sequence denaturation, primer annealing and Taq extension with suitable temperatures
  • explain primer specificity, dNTP supply and thermostable Taq polymerase in each cycle
  • calculate copy number or starting-DNA ratios from doubling and threshold-cycle data

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