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CAIE A-Level Biology 19.1.3 Sources of Genes

Practise comparing genes isolated from donor DNA with cDNA made from mRNA and sequences synthesised chemically from known nucleotide information.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • outline gene isolation from donor DNA using a suitable restriction endonuclease
  • trace mRNA through reverse transcriptase and DNA polymerase to double-stranded cDNA
  • explain why intron-free cDNA, unlike human genomic DNA, can be expressed in bacteria

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