CAIE IGCSE Biology 21.3.3 Process of Genetic Modification Using Questions
Practise sequencing restriction enzymes, sticky ends, DNA ligase and recombinant plasmids to make human proteins in bacteria.
- Syllabus
- 2026–2028
- Course
- Biology 0610
Practise sequencing restriction enzymes, sticky ends, DNA ligase and recombinant plasmids to make human proteins in bacteria.
Bacteria are useful in biotechnology and genetic engineering.
Fig. 6.1 shows a photomicrograph of a bacterium.
Fig. 6.1
Genetically modified bacteria can produce human insulin.
Insulin is a protein.
Describe the process of using bacteria in genetic engineering to produce human proteins.
ref. to (human) gene / DNA that codes for (human) protein ;
(human) DNA / gene, is, identified / isolated ;
DNA / gene / plasmid, cut (out) using restriction enzymes ;
forming, complementary / sticky, ends ;
DNA / gene / plasmid, cut with the same restriction enzymes ;
formation of recombinant, DNA / plasmid ;
into plasmid
(DNA) ligase used to join plasmid and, gene / DNA ;
plasmids (with gene) inserted into bacteria ;
bacteria (with the plasmid), replicate / reproduce / multiply ;
AVP ;
e.g. purification / identification of transformed bacteria /
procedures must be in the correct sequence