CAIE IGCSE Biology 21.3.3 Process of Genetic Modification Using Questions

Practise sequencing how a human gene is isolated, joined to a bacterial plasmid and expressed after recombinant bacteria multiply.

Syllabus
2026–2028
Course
Biology 0610

Exam points

  • use the same restriction enzyme to cut the human gene and plasmid, forming complementary sticky ends
  • join the gene and plasmid with DNA ligase to form a recombinant plasmid
  • sequence plasmid insertion, bacterial multiplication and expression of the human protein

CAIE IGCSE Biology 21.3.3 Process of Genetic Modification Using Questions question 1

[Maximum number: 5]

Bacteria are useful in biotechnology and genetic engineering.
Fig. 6.1 shows a photomicrograph of a bacterium.

Fig. 6.1

Fig. 6.1

Genetically modified bacteria can produce human insulin.

Insulin is a protein.

Describe the process of using bacteria in genetic engineering to produce human proteins.

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