Explore how treatment, tissue and time-course evidence reveals gene activation and how controls and uncertainty constrain transcriptional conclusions in IB Biology HL D1.2.4.
Auxin can be used to promote the development of roots from stem and leafy cuttings in some plants. In a study into the distribution of auxin in the development of these roots, scientists measured the amount of auxin in different leaves of a shoot tip of Petunia hybrida.
The figure indicates the numbering of leaves on the shoot, from L1 as the youngest and smallest to L6 as the largest and oldest leaf. The developmental stage of L5 and L6 was very similar, so L5 was not analysed. The stem base is the lowest part of the cutting where roots may form.
The graph shows the auxin concentration in the different leaves.
The scientists wanted to know whether the accumulation of auxin over time in the stem base of the controls affected expression of the GH3 gene, known to have a role in growth regulation in different plants. The technique that was used to quantify the level of transcription of the GH3 gene was Northern blotting. In this procedure the darkness and thickness of the band is an indicator of the level of transcription of a particular gene. The image shows the result of the Northern blot from 2 hours to 24 hours after cutting.
Compare the pattern of GH3 transcription with the pattern of auxin concentration in the stem base control cuttings. You may use the table provided to help you to record the patterns before you compare them. (Please note: a simple comparison in the table will not gain marks)
The scientists concluded that auxin activates the transcription of the GH 3 gene. Using the information on the auxin concentration in the stem base in the graph on page 4 and the Northern blot, evaluate whether this conclusion is supported.