a. enzymes are globular proteins with an active site (for catalysis);
b. substrates must bind to the active site to be catalysed;
c. description of induced fit mechanism / substrate can cause active site to change shape;
d. conformational changes/changes in tertiary structure do not allow substrate to bind to active site / enzyme is denatured;
e. denaturation decreases enzyme activity / enzymes no longer work;
f. high temperatures/temperatures above the optimum break bonds in the enzymes / cause conformational changes to active site / denature enzymes;
g. below the optimum temperature enzymes may be inhibited OR increased temperature increases kinetic energy, increasing enzyme activity (because of more collisions) up to optimum temperature;
h. (changes in) pH cause changes in charges /alteration of (ionic/ H) bonds (between amino acids) / affect shape of active site / the enzyme becomes denatured (at higher or lower pH values than the optimum)
i. non-competitive inhibitors bind to an allosteric site;
j. (non-competitive inhibitors) cause disruption of hydrogen/ionic/disulfide/hydrophobic interactions;
Marking guidance: