Measure substrate loss or product formation per unit time, interpret initial rates from amylase or catalase graphs, and assess reliability with replicates and controls in IB Biology HL C1.1.9.
Laccase is an enzyme that has the ability to decompose synthetic dyes used in the production of clothing and thus could potentially help reduce synthetic dye pollution.
An experiment was performed in which laccase was immobilized in alginate beads to determine the effectiveness of laccase immobilization in decolorizing the dye crystal violet. The graph shows the effect of laccase concentration on relative decolorization activity of the immobilized laccase. Error bars represent ± SD.
Deduce the significance of overlapping error bars in the data for laccase concentrations from to .
Discuss the variables that need to be controlled in this investigation.
Suggest how the percentage of decolorization could be obtained experimentally.