IB Biology HL C1.1 Enzymes and Metabolism Questions

Practise IB Biology HL C1.1 by explaining enzyme specificity, metabolic reactions, rate evidence, inhibition and feedback control in biological pathways.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam points

  • Explain enzyme catalysis through activation-energy reduction and relate active-site specificity to substrate binding and induced fit.
  • Interpret metabolic pathways and classify enzyme-controlled reactions, including condensation, hydrolysis and oxidation, as anabolic or catabolic.
  • Explain how tertiary-structure and active-site changes affect enzyme binding and activity.
  • Interpret enzyme-rate graphs and data for temperature, pH, substrate concentration, optima and saturation; evaluate assay reliability.
  • Interpret enzyme energy profiles to identify reduced activation energy without a change in reactant or product energy.
  • Distinguish intracellular and extracellular enzymes using digestive-enzyme examples and pepsinogen activation.
  • Relate basal metabolic rate and heat production to climate, body mass and surface-area-to-volume ratio.
  • Distinguish linear from cyclical metabolic pathways by whether an intermediate is regenerated.
  • Compare competitive and non-competitive inhibition using binding site and substrate-response evidence.
  • Explain feedback inhibition by end-product binding to an earlier pathway enzyme, including isoleucine synthesis.

Question 1

[Maximum number: 4]

Outline the action of enzymes.

Question 2

[Maximum number: 6]

Question (a)

(a)

Identify the following processes as either anabolism or catabolism by placing a tick ( ✓ ) in the correct box.

Table for Question (a) — IB Biology HL
[ 2 ]

Question (b)

(b)

Outline the importance of enzymes to metabolic processes.

[ 4 ]

Question 3

[Maximum number: 1]

What is an example of anabolism?

A

Formation of peptides after protein digestion

B

Formation of glucose and fructose from sucrose

C

Formation of maltose from two glucose molecules

D

Formation of glycerol and fatty acids from triglycerides

Question 4

[Maximum number: 4]

Outline the importance of enzymes to metabolic processes.

Question 5

[Maximum number: 1]

Define the active site of an enzyme.

Question 6

[Maximum number: 6]

Describe the lock and key model of enzyme activity and how the induced fit model extends it.

Question 7

[Maximum number: 2]

Explain how the active site promotes enzyme-substrate specificity.

Question 8

[Maximum number: 3]

Cholinergic synapses use acetylcholine as their neurotransmitter. They are widespread in the body, passing on signals to muscle cells. These synapses are affected by neonicotinoid pesticides.

The diagrams, which are not drawn to scale, show the synapse between two neurons and a detail of the synaptic cleft.

Figure for Question 8 — IB Biology HL

Explain what happens to an enzyme if there is a change of pH .

Question 9

[Maximum number: 6]

Laccase is an enzyme that has the ability to decompose synthetic dyes used in the production of clothing and thus could potentially help reduce synthetic dye pollution.

An experiment was performed in which laccase was immobilized in alginate beads to determine the effectiveness of laccase immobilization in decolorizing the dye crystal violet. The graph shows the effect of laccase concentration on relative decolorization activity of the immobilized laccase. Error bars represent ± SD.

Figure for Question 9 — IB Biology HL

Question (a)

(a)

Deduce the significance of overlapping error bars in the data for laccase concentrations from 3mgml−13 \mathrm{mg} \mathrm{ml}^{-1} to 4mgml−14 \mathrm{mg} \mathrm{ml}^{-1}.

[ 1 ]

Question (b)

(b)

Discuss the variables that need to be controlled in this investigation.

[ 2 ]

Question (c)

(c)

Suggest how the percentage of decolorization could be obtained experimentally.

[ 3 ]

Question 10

[Maximum number: 1]

(a) The image shows a cell in a section of an onion root tip seen under a light microscope.

Figure for Question 10 — IB Biology HL

The graph shows energy levels throughout an uncatalysed reaction. Draw a curve to show how the action of an enzyme would affect this reaction.

Figure for Question 10 — IB Biology HL

Question 11

[Maximum number: 1]

What is an example of an intracellular enzyme-catalysed reaction?

A

Chemical digestion in the gut

B

Glycolysis in cellular respiration

C

Blood clotting in the circulatory system

D

Recycling of acetylcholine in a synapse

Question 12

[Maximum number: 4]

Common bottlenose dolphins, Tursiops truncatus, are marine mammals of temperate and tropical oceans throughout the world, where they feed on fish, squid and crustaceans.

Research has been carried out on a population of these dolphins in Sarasota Bay, Florida. The table shows the mean sea surface temperature in Sarasota Bay and catch per unit effort (CPUE) in winter and summer. A large CPUE indicates it would take little effort for the dolphin to catch a lot of prey.

Sarasota Bay winter/summer data: mean sea-surface temperature 22.6/28.7°C; catch per unit effort 110.5/663.2.

Question (a)

(a)

Explain the differences in BMR between desert and polar mammals up to a mass of 103 g10^{3} \mathrm{~g}.

[ 2 ]

Question (b)

(b)

Above a body mass of 10 kg , there is little difference in BMR between desert and polar mammals.

Suggest reasons for this.

[ 2 ]

Question 13

[Maximum number: 1]

Laccase is an enzyme that has the ability to decompose synthetic dyes used in the production of clothing and thus could potentially help reduce synthetic dye pollution.

An experiment was performed in which laccase was immobilized in alginate beads to determine the effectiveness of laccase immobilization in decolorizing the dye crystal violet. The graph shows the effect of laccase concentration on relative decolorization activity of the immobilized laccase. Error bars represent ± SD.

Figure for Question 13 — IB Biology HL

Distinguish between processes involved in cyclical and linear metabolic pathways.

Question 14

[Maximum number: 1]

The dashed line shows the relationship between the activity of an enzyme and the concentration of its substrate. Which curve shows the effect of a non-competitive inhibitor on this relationship?

Figure for Question 14 — IB Biology HL

Question 15

[Maximum number: 4]

Interactions in biological systems may have harmful or beneficial effects.

Explain, using an example, how competitive inhibitors interfere with enzyme activity.

Question 16

[Maximum number: 4]

Feedback mechanisms are used in living organisms both to promote and respond to change.

Explain the regulation of metabolic pathways by end-product inhibition.

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