IB Biology SL D2.3 Water Potential Question Bank
Practise IB Biology SL D2.3 by explaining osmosis and predicting plant and animal cell responses in water-potential experiments.
- Syllabus
- First assessment 2025
- Course
- Biology SL
- Level
- SL
Practise IB Biology SL D2.3 by explaining osmosis and predicting plant and animal cell responses in water-potential experiments.
A study was done on the osmolarity of semen and sperm movement (motility).

The same study showed that blood plasma has a lower osmolarity than semen. Explain how that would affect the movement of water between blood plasma and semen.
ii
water would move from blood plasma to the semen as it follows the concentration gradient / from low osmolarity to higher;
Must include reason/explanation.
Explain a change that would take place in sperm placed in a hypotonic solution.
a. water will enter (by osmosis) causing sperm to swell/enlarge/burst;
b. water will enter (by osmosis) causing sperm to become more mobile as osmolarity decreases;
Marking guidance:
Do not accept turgidity.
Must show movement of water.
The electron micrograph shows the surface membrane of an animal cell.

Explain the reason that animal cells and tissues under investigation must be maintained in solutions with the same osmolarity.
a. to prevent osmosis;
b. cells placed in the in the incorrect osmolarity might swell/burst/shrink;
c. hypotonic solution would cause water to enter cells/tissues;
d. hypertonic solutions would cause water to leave cells/tissues;
e. water loss would hinder (metabolic) reactions in cell cytoplasm
OR
distort appearance of the cells (for the investigation);
Mp c and d must indicate direction of water movement
Marking guidance:
Accept wtte for hypo- and hyper-tonic
3 max
An experiment was carried out to determine the change in mass due to osmosis. Potato cubes were soaked in different concentrations of sodium chloride for 12 hours. The table shows the mean data for the mass of potato before and after the 12-hour period.

Outline the conditions necessary for osmosis to occur.
a. partially/semi permeable/selective membrane (to water);
b. solute molecules/ions cannot pass through membrane;
c. different solute/water concentrations/concentration gradients/osmolarity;
d. suitable temperature (for osmosis to occur);
2 max
Explain how the data may be used to determine the solute concentration of the potato tissue.
a. calculate percentage difference in mass;
b. plot (percentage change) mass against concentration;
c. concentration of solutes in potato tissues corresponds to 0 / zero / no mass change OR
where the line (of best fit) crosses the mass change at zero is the potato tissue concentration;
Marking guidance:
Accept the correct calculation for percentage (final mass - initial, divided by initial and times 100).
Accept annotated graph.
2 max
Estimate the solute concentration of the potato tissue.
Any number from 0.31 moldm−3 to 0.39 moldm−3;
Units required.