CAIE IGCSE Biology 12.1.2 Temperature & Respiration
Practise investigating how temperature changes respiration rate in yeast and seeds using gas-volume data, graphs and respirometers.
- Syllabus
- 2026–2028
- Course
- Biology 0610
Practise investigating how temperature changes respiration rate in yeast and seeds using gas-volume data, graphs and respirometers.
One problem with using biomass in the process shown in Fig. 4.1 is that the breakdown stage produces a sugar called xylose and ethanoic acid. Yeast cannot use xylose, and ethanoic acid is toxic to yeast.
Scientists genetically engineered a type of yeast that can use xylose and ethanoic acid.
Fig. 4.2 shows the results of one of the trial experiments done by the scientists using their new genetically engineered yeast.

Fig. 4.2
The experiment was done at 30∘C.
The scientists repeated the experiment at 20∘C.
Predict the results that you would expect for the concentration of ethanol.
one from:
(s)lower rate of increase of ethanol / less ethanol (in same time) ; take longer to reach same concentration of ethanol ; line(s) / gradient(s) (on graph), less steep ;