IB Biology SL D1.1.4 Pcr and Gel Electrophoresis Questions
Explore PCR temperature cycles, Taq polymerase, DNA-fragment separation and gel-band interpretation in IB Biology SL D1.1.4.
- Syllabus
- First assessment 2025
- Course
- Biology SL
- Level
- SL
Exam points
- Explain PCR cycles using DNA denaturation, primer annealing and Taq-polymerase extension to amplify a selected DNA sequence.
- Explain how gel electrophoresis separates DNA fragments by charge and size, and interpret band position or pattern.
- Use PCR or gel controls, cycle conditions and band evidence to evaluate an amplification or DNA-analysis result.
IB Biology SL D1.1.4 Pcr and Gel Electrophoresis Questions question 1
[Maximum number: 2]
Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA.
strands of DNA (fragments) split/denatured with heat
complementary nucleotides added to split stands (when cooling)
with the use of (DNA) polymerase (and primers)
process/heating and cooling cycle is repeated (until enough DNA is obtained)
example of PCR application (e.g. paternity cases or forensic investigations)
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