IB Biology SL 1.1.4 PCR and gel electrophoresis Question Bank
Practise IB Biology SL 1.1.4 by describing PCR amplification and separating DNA fragments by gel electrophoresis.
- Syllabus
- First assessment 2025
- Course
- Biology SL
- Level
- SL
Exam points
- Outline the denaturation, primer-binding and extension stages of PCR.
- Explain why Taq DNA polymerase is suitable for repeated high-temperature cycles.
- Explain how gel electrophoresis separates DNA fragments by size in an electric field.
D1.1.4—PCR and gel electrophoresis question 1
[Maximum number: 2]
Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA.
strands of DNA (fragments) split/denatured with heat;
complementary nucleotides added to split stands (when cooling);
with the use of (DNA) polymerase (and primers); process/heating and cooling cycle is repeated (until enough DNA is obtained);
example of PCR application; (e.g. paternity cases or forensic investigations)
Remember, up to TWO "quality of construction" marks per essay.
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