IB Biology SL 8.1.9 Enzyme activity Question Bank

Practise IB Biology SL 8.1.9 by measuring product formation or substrate loss and controlling variables such as pH, temperature and enzyme concentration.

Exam points

Choose product or substrate measurements that quantify enzyme activity over time.
Identify controlled variables and use error bars or replicates to judge reliability.

[Maximum number: 2]

The enzyme beta-galactosidase hydrolyses lactose to release glucose and fructose. A study was carried out to determine how acidity affects the activity of a beta-galactosidase enzyme, extracted from the fungus Penicillium simplicissimum.

Figure for Question C1.1.9—Measuring enzyme reactions question 1 — IB Biology SL

Outline the measurements which would need to be taken to determine the activity of the beta-galactosidase at different pH values.

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C1.1.9—Measuring enzyme reactions question 1