IB Biology SL C1.1.9 Measuring Enzyme Reactions Questions

Practise IB Biology SL 8.1.9 by measuring product formation or substrate loss and controlling variables such as pH, temperature and enzyme concentration.

Exam points

Choose product formation or substrate loss measurements per unit time, using an initial rate to quantify enzyme activity.
Identify controlled variables, replicates and error bars, then judge reliability of enzyme-assay results.
Calculate or interpret reaction rates from curves and data, and propose practical improvements to the assay.

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Study C1.1.9—Measuring enzyme reactions conceptsOpen the corresponding Concept lesson for explanations, examples and exam connections.

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The enzyme beta-galactosidase hydrolyses lactose to release glucose and fructose. A study was carried out to determine how acidity affects the activity of a beta-galactosidase enzyme, extracted from the fungus Penicillium simplicissimum.

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Outline the measurements which would need to be taken to determine the activity of the beta-galactosidase at different pH values.

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IB Biology SL C1.1.9 Measuring Enzyme Reactions Questions question 1