IB Biology SL C1.1.9 Measuring Enzyme Reactions Questions

Measure substrate loss or product formation per unit time, interpret initial rates from amylase or catalase graphs, and assess reliability with replicates and controls in IB Biology SL C1.1.9.

Exam points

Measure enzyme activity as substrate loss or product formation per unit time.
Use an initial rate from amylase or catalase assay graphs to compare enzyme activity.
Use replicates and controlled variables when measuring enzyme reactions to assess result reliability.

[Maximum number: 2]

The enzyme beta-galactosidase hydrolyses lactose to release glucose and fructose. A study was carried out to determine how acidity affects the activity of a beta-galactosidase enzyme, extracted from the fungus Penicillium simplicissimum.

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Outline the measurements which would need to be taken to determine the activity of the beta-galactosidase at different pH values.

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IB Biology SL C1.1.9 Measuring Enzyme Reactions Questions question 1