D3.2.2—Genetic crosses in flowering plants

Genetic crosses in flowering plants use parental genotypes, gametes and offspring ratios to infer inheritance patterns in inheritance problems in inheritance problems.

Syllabus
First assessment 2025
Objective
D3.2.2
Level
SL

Exam analysis

Chance of appearing2%of analysed past papers
Latest appearanceMay 2023
Most common paperPaper1
Typical marks1–3

Common command terms

  • Identify

Recent exam appearances

May 2023Paper2 ["SL"] · TZ22(b)[ 3 ]D3.2.2—Genetic crosses in flowering plants
November 2020Paper1 ["SL"] · TZ014[ 1 ]D3.2.2—Genetic crosses in flowering plants
November 2014Paper1 ["SL"] · TZ016[ 1 ]D3.2.2—Genetic crosses in flowering plants
Practice this objective

Coverage 2014–2023 · Updated 16 Jul 2026

Use Flowering Plants to Perform Genetic Crosses

A flowering-plant cross transfers pollen carrying male gametes to a stigma so fertilization can combine known parental alleles.

Generation What to do and record
P Choose parents with known contrasting traits and control which pollen reaches the stigma
F1 Grow the first filial offspring and record their phenotype(s)
F2 Cross or self-pollinate suitable F1 plants, then compare observed offspring with a Punnett-grid prediction

Pollen is the practical source of male gametes; female gametes are inside ovules in the ovary. Pea flowers can self-pollinate, which helps maintain pure-breeding lines and produce controlled F2 generations.

Cross two pure-breeding parents with contrasting traits, record a uniform F1, then self-pollinate the F1 and compare the F2 counts with the predicted genotype and phenotype ratios.

A Punnett grid predicts probabilities, not exact counts. Controlled crosses are used in crop and ornamental breeding, but pollination is transfer of pollen, not fertilization itself.

Genetic crosses in flowering plants

Assessment in practice

1 marks
How it is assessed

This objective is assessed through structured response, commonly using Identify.

Command terms

Identify

What earns marks

Build the answer around this relationship: Parental genotypes determine the gametes available in a cross.

Representative question

Question 1

[Maximum number: 3]

L. purpureus can have purple or white flowers. Two pure-breeding varieties were crossed: HA 4 with white flowers and GL 424 with purple flowers. All of the F1F_{1} plants had purple flowers. The F1F_{1} plants were self-pollinated to produce an F2F_{2} generation. There were 97 plants with purple flowers and 38 plants with white flowers in the F2F_{2} generation.

Using a Punnett grid, explain the results of this cross.

Retrieve the Core Inheritance Route

Core D3.2 is secure when the student can move from allele rules into predictions and evidence: gametes form genotypes, genotypes can produce phenotypes, different dominance patterns need different notation, and pedigrees or plots require evidence-based interpretation.

  • haploid gametes carry one allele and fertilization restores a diploid genotype
  • dominance, codominance, incomplete dominance, environment, and plasticity affect the observed trait
  • PKU, ABO, sex determination, and haemophilia use different inheritance rules and notation
  • pedigrees infer inheritance patterns and box plots summarize continuous variation

Solve Core Inheritance Questions

Core inheritance exam questions reward disciplined reasoning. First identify the inheritance rule, then write the correct notation or evidence, then state the phenotype, ratio, or conclusion. This prevents the common mistake of writing definitions without solving the genetic problem.

  • Use allele and genotype notation correctly for monohybrid, ABO, PKU, haemophilia, and sex-determination contexts.
  • Connect genotype, dominance pattern, environment, or plasticity to phenotype.
  • Use pedigree or box-plot evidence to justify an inheritance or variation conclusion.

Concept essentials

  • Parental genotypes determine the gametes available in a cross.
  • Offspring ratios can reveal whether a parent is homozygous or heterozygous.
  • Controlled pollination in plants allows inheritance predictions to be tested.