D2.3 Water potential
Water potential explains osmosis, solute effects, plant tissue changes, cell swelling, plasmolysis and isotonic medical conditions in living systems and cells.
- Syllabus
- First assessment 2025
- Topic
- D2.3
- Level
- SL
Water potential explains osmosis, solute effects, plant tissue changes, cell swelling, plasmolysis and isotonic medical conditions in living systems and cells.
Solvation is the attraction of water molecules to ions or polar molecules, forming a hydrated environment around the solute.
Water is polar: its partial charges orient toward charged or polar regions. These attractions separate particles and keep them dispersed, which helps cells transport dissolved substances.
Check: solute charge or polarity; water orientation; particle separation; resulting solution.
When sodium chloride dissolves, oxygen ends of water face Na+ while hydrogen ends face Cl−, forming hydration shells.
Non-polar solutes are not automatically soluble in water; polarity and the relevant intermolecular attractions matter.
Water moves from higher water potential to lower water potential through a selectively permeable pathway.
The gradient is a difference in the tendency of water to leave a region. A membrane may let water cross while restricting solute, so net movement is osmosis rather than solute diffusion.
Predict direction by comparing the two water potentials before deciding which particles cross.
If a cell interior is −0.4 MPa and the surrounding solution is −0.1 MPa, water enters the cell because −0.1 MPa is higher.
Net movement can stop at equilibrium; individual water molecules still move in both directions.
This objective is assessed through structured response, multiple choice, commonly using Explain / Outline.
Explain / Outline
Build the answer around this relationship: Osmosis requires a partially permeable membrane.
Saying solute moves by osmosis instead of water.
Representative question
Outline the conditions necessary for osmosis to occur.
a. partially/semi permeable/selective membrane (to water);
b. solute molecules/ions cannot pass through membrane;
c. different solute/water concentrations/concentration gradients/osmolarity;
d. suitable temperature (for osmosis to occur);
2 max
Osmosis is the net movement of water across a partially permeable membrane in response to a water-potential difference.
Water crosses more readily than many solutes. As water enters or leaves, cell volume and internal pressure change until the gradient is reduced or opposing forces balance it.
Classify a case by membrane selectivity, initial water potentials, and whether volume rises or falls.
A cell placed in a solution with lower water potential loses water, so its mass decreases even though solute particles do not cross the membrane.
Do not say water moves toward ‘more solute’ without checking the full water potential and membrane conditions.
This objective is assessed through structured response, commonly using Explain.
Explain
Build the answer around this relationship: Hypotonic external solutions cause net water entry into cells.
Representative question
Explain the reason that animal cells and tissues under investigation must be maintained in solutions with the same osmolarity.
a. to prevent osmosis;
b. cells placed in the in the incorrect osmolarity might swell/burst/shrink;
c. hypotonic solution would cause water to enter cells/tissues;
d. hypertonic solutions would cause water to leave cells/tissues;
e. water loss would hinder (metabolic) reactions in cell cytoplasm
OR
distort appearance of the cells (for the investigation);
Mp c and d must indicate direction of water movement
Marking guidance:
Accept wtte for hypo- and hyper-tonic
3 max
Water potential differences change plant-cell turgor and therefore tissue firmness and growth.
Water entering a vacuolated cell raises turgor pressure against the wall; water leaving reduces turgor and can pull the membrane from the wall. Tissue-level effects add across many cells.
Link condition to tissue outcome: external potential; water movement; turgor; visible change.
A leafy shoot in a concentrated solution loses water, cells become flaccid, and the leaves droop because turgor falls.
Wilting is not proof that every cell has died; restoring water can recover turgor if damage is limited.
This objective is assessed through experimental design, commonly using Identify / Describe / Explain.
Identify / Describe / Explain / Suggest / Evaluate / Outline
Build the answer around this relationship: Percentage change in mass shows relative water gain or loss.
Using raw mass change instead of percentage change when initial masses differ.
Representative question
Student osmosis experiments often involve putting plant tissue such as potato cylinders in several salt solutions of different concentrations and measuring the mass before and after immersion. Outline how data collected from such an experiment could be used to estimate the osmolarity of the plant tissues.
a. (percentage) change in mass can be used;
b. plot concentration of solution on x-axis AND (percentage) change in mass on y-axis
OR
gain in mass means hypotonic (solution)/vice versa;
c. point where data line intercepts zero (\%) change in mass is osmolarity/osmotic concentration (of tissue)
OR
no change in mass means isotonic (solution);
Answer must refer to mass of (cylinders/tissue).
a. Accept increase/decrease.
b. Both axes needed for [1]
Accept sketch of graph correctly drawn and annotated e.g.
2 max
A cell without a rigid wall changes volume when osmosis alters its water content.
The plasma membrane can deform, but it cannot provide the same mechanical restraint as a cell wall. Water entry raises internal pressure; excessive entry can rupture the membrane.
Use the membrane boundary and water direction to predict whether volume rises, falls, or risks lysis.
A red blood cell in a hypotonic solution takes in water and may haemolyse because no cell wall limits expansion.
The outcome also depends on solute concentrations, membrane permeability, and whether active regulation is available.
This objective is assessed through structured response, multiple choice, commonly using Deduce / Outline / Predict.
Deduce / Outline / Predict / Explain / Describe / Suggest
Build the answer around this relationship: Hypotonic solutions can cause animal cells to swell or lyse.
Calling crenated animal cells turgid, a term that applies to walled plant cells.
Representative question
Explain the effect of placing red blood cells in distilled water (0.000 M NaCl).
a. red blood cells are hypertonic/more concentrated/have lower water potential/higher solute concentration (than distilled water)/vice versa;
b. water moves into cells by osmosis;
c. water moves from an area of higher water potential/concentration/ solution (in water) to lower water potential/concentration (in cell) OR water moves from a more dilute solution to a more concentrated solution
OR
water moves from hypotonic to hypertonic solution;
d. through a (selectively) permeable membrane;
e. cells swell and (eventually) burst/ complete/100\% hemolysis;
3 max
A cell wall resists expansion when water enters, converting osmotic water uptake into turgor pressure.
The wall’s rigidity balances the inward tendency of water. If water leaves, pressure falls and the membrane can pull away from the wall, producing plasmolysis.
Distinguish wall restraint from membrane transport: water direction first, then pressure and shape.
A plant cell in dilute solution becomes turgid rather than bursting because the wall pushes back as the vacuole expands.
A wall prevents unlimited swelling but does not stop osmosis or guarantee that a severely dehydrated cell survives.
This objective is assessed through structured response, commonly using Outline / State / Explain.
Outline / State / Explain
Build the answer around this relationship: Water entry can make plant cells turgid.
Saying plant cells burst in hypotonic solution ignores the protective cell wall.
Representative question
Explain the process of osmosis with reference to its effects on plant cells.
a. osmosis is water moving through a partially/semi permeable membrane/cell membrane/aquaporins;
b. osmosis is a form of simple diffusion
OR
water travels down the (water) concentration gradient
OR
osmosis is passive;
c. the solute concentration determines the direction of net movement of water;
d. plant cells in hypotonic solutions will take in water;
e. they will swell/are turgid/develop turgor pressure (in hypotonic solutions);
f. cell wall prevents the plant cells from bursting due to water pressure (in hypotonic solutions);
g. plant cells in hypertonic solutions will lose water;
h. they will shrink/get smaller/flaccid (in hypertonic solutions);
i. the cell membrane will pull away from the cell wall/plasmolysis (in hypertonic solutions);
j. isotonic solutions are the same concentration as inside the plant cells/cytoplasm/cell sap;
k. plant cells will have no net change (in isotonic solutions)/water moves equally in and out of the cell/dynamic equilibrium;
7
Marking guidance:
max
Medical fluids are chosen so their effective water potential does not cause harmful net water movement across cell membranes.
If an infused solution is too dilute, cells gain water; if too concentrated, cells lose water. Matching osmotic conditions protects cell volume while solutes are delivered.
Evaluate a fluid by predicting water movement into or out of cells before considering its clinical use.
An isotonic saline infusion can replace extracellular fluid without making red blood cells swell or shrink appreciably.
‘Isotonic’ is relative to the cells and relevant solutes; filtration, metabolism, and patient condition can change the outcome.
This objective is assessed through structured response, multiple choice, commonly using Explain.
Explain
Build the answer around this relationship: Isotonic fluids prevent net water movement into or out of body cells.
Representative question
Explain the need for isotonic conditions in human blood plasma and tissue fluid.
a. isotonic is same water potential/solute potential/osmotic potential/solute concentration;
b. (isotonic conditions) prevent (net) movement of water by osmosis;
c. hypotonic plasma/tissue fluid causes water entry to blood/body cells;
d. swelling/bursting of cells in (hypotonic plasma/tissue fluid);
e. hypertonic plasma/tissue fluid causes water to exit cells;
f. shrinkage/crenation/loss of volume/OWTTE;
g. blood cells bathed in plasma;
h. tissue fluid released by capillaries and then reabsorbed/flows between cells in tissues;
In mark point c and mark point e
Marking guidance:
accept 'higher/less negative
water potential' instead of
hypotonic and accept
'lower/more negative water
potential’ instead of hypertonic.
4
max
Water forms hydration shells around ions and polar solutes; hydrogen bonding and charge attraction reduce free water movement. Water moves by osmosis across partially permeable membranes from hypotonic/lower solute solutions toward hypertonic/higher solute solutions. Osmosis direction depends on internal and external solute concentration; isotonic conditions have dynamic water movement but no net osmosis. Plant tissue changes mass or length in sucrose solutions; percentage change graphs estimate isotonic or osmotic concentration. Animal cells can lyse in hypotonic solutions and crenate in hypertonic solutions; freshwater protists use contractile vacuoles to expel excess water. Plant cells become turgid in hypotonic solutions as vacuoles swell; hypertonic solutions cause flaccidity and plasmolysis from water loss. Isotonic saline prevents harmful water gain or loss in body cells; IV fluids and transplant organ baths must match tissue osmotic concentration.