IB Biology HL 3.1.20 Auxin efflux carriers Question Bank
Practise IB Biology HL 3.1.20 by applying auxin efflux carriers concepts to exam-style questions.
- Syllabus
- First assessment 2025
- Course
- Biology HL
- Level
- HL
Practise IB Biology HL 3.1.20 by applying auxin efflux carriers concepts to exam-style questions.
Auxin can be used to promote the development of roots from stem and leafy cuttings in some plants. In a study into the distribution of auxin in the development of these roots, scientists measured the amount of auxin in different leaves of a shoot tip of Petunia hybrida.
The figure indicates the numbering of leaves on the shoot, from L1 as the youngest and smallest to L6 as the largest and oldest leaf. The developmental stage of L5 and L6 was very similar, so L5 was not analysed. The stem base is the lowest part of the cutting where roots may form.

The graph shows the auxin concentration in the different leaves.

N-1-naphthylphthalamic acid (NPA) is an inhibitor used to block auxin transport. NPA was sprayed onto the leaves of a set of cuttings for 14 days. Development of the roots in control (non-treated) and NPA-treated cuttings was measured 14 days after taking the cuttings. The table shows the influence of NPA on rooting.

Analyse the effect of NPA on the formation of roots.
a. NPA decreased the «mean» number of roots per rooted cutting «by about 5× »
b. NPA decreased the «mean» length per root «by more than half»
c. NPA decreased the «mean» total root length per planted cutting «to about 2 % of control»
d. NPA inhibited the formation of roots
OR
decreased all three measures
Marking guidance:
Accept other correct statements of overall changes in values.
The word "mean" is not required. OWTTE
OWTTE
2 max
The scientists also measured the changes in auxin concentration in L6 and the stem base during the early period of root formation. They recorded the concentration in the control and NPA-treated cuttings for 24 hours after taking the cuttings.

Compare and contrast the changes in auxin concentration in the stem base over time for the control and NPA-treated cuttings.
a. both decrease up to 6 hours/initially
b. NPA-treated decrease more/at a faster rate than control «up to 6 hours»
c. after 6 hours, control increases while NPA treated continues to fall
2 max
Deduce the effect of NPA on auxin transport between L6 and the stem base.
ii
a. NPA «appears to have» no effect on concentrations/transport of auxin in L6 as control and NPA-treated remain at same «low» level
b. NPA «probably» inhibits the auxin efflux pumps/transport «in the leaves» as the levels drop in NPA-treated in stem base «but not in control»
c. the transport of auxin to the stem base must occur from younger leaves
OR
L6 is not the source of auxin in the stem base
d. NPA inhibits the auxin pumps/transport «in the leaves» as the levels drop in NPA-treated in stem base
OWTTE
A valid reason must be given for the mark.
OWTTE
A valid reason must be given for the mark.
2 max