Edexcel IGCSE Biology 2.9 food tests
Practise food tests by choosing safe methods and interpreting Benedict's, iodine, Biuret and emulsion colour changes in samples.
- Syllabus
- First assessment 2019
- Course
- Biology 4BI1
Practise food tests by choosing safe methods and interpreting Benedict's, iodine, Biuret and emulsion colour changes in samples.
Pineapple juice contains a protease called bromelain.
A student uses this method to investigate the digestion of solid gelatine protein by bromelain.
- place solid gelatine protein into a test tube up to a height of 5 cm
- mix 5 cm3 pineapple juice with 1 cm3 of pH 4 buffer
- place 1 cm3 of the pineapple juice and buffer solution on top of the gelatine
- leave for one hour in a water bath set to 37∘C
- measure the height of the solid gelatine and use it to calculate the volume of gelatine that has been digested
Repeat the method three more times.
The diagram shows part of the student's method.

Describe how to test for the presence of protein.
A description that makes reference to the following.
- add biuret (reagent) / add biuret A and biuret B / sodium hydroxide & copper sulfate
- turns lilac / purple / pink / mauve / eq
correct,
alternative
tests e.g. ninhydrin test goes red /
brown
xanthoproteic test goes
yellow