Edexcel IGCSE Biology 2.12 enzyme activity practical
Practise enzyme practical design by planning temperature ranges, repeats, water baths, mean calculations and accuracy improvements.
- Syllabus
- First assessment 2019
- Course
- Biology 4BI1
Exam points
- Design enzyme practicals with temperature ranges, controlled variables and repeat measurements.
- Calculate means or percentage changes from tables of time or oxygen-volume data.
- Improve accuracy with water baths, equilibration time and smaller temperature intervals.
2.12 Enzyme activity practical question 1
[Maximum number: 6]
Biological washing powders contain protease enzymes to digest proteins in clothing stains.
Scientists have produced recombinant plasmids containing a gene for a protease enzyme that works at high temperatures. The plasmids have been inserted into bacteria so that the bacteria produce the protease enzyme.
Design an investigation to find the best temperature for removing protein stains by using washing powder that contains protease enzyme.
Include experimental details in your answer and write in full sentences.
An answer that makes reference to the following points:
- C: range of at least three temperatures / use a wide range of temperatures
- O: same concentration of enzyme / mass of washing powder / type of washing powder / type of enzyme / eq
- R: repeats
- M1: measure area / size of stain / mass of stain / colour of stain / mass of protein / eq
- M2: stated time period
- S1 same pH / water volume / washing movements / same type of material / clothing / size of material / eq
- S2 same mass of protein / area of protein / type of protein stain / same blood / same named protein / eq
Allow amount of washing powder
Ignore amount of stain
Allow M1 and M2 for time taken to remove stain
Allow times between 10 minutes and 24 hours
Allow same concentration of stain
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