Edexcel IGCSE Biology 5 use of biological resources
Practise applying biological resource ideas to food production, fermentation, selective breeding, genetic modification and cloning contexts.
- Syllabus
- First assessment 2019
- Course
- Biology 4BI1
Practise applying biological resource ideas to food production, fermentation, selective breeding, genetic modification and cloning contexts.
The diagram shows a flower with some structures labelled.

A farmer has two varieties of a plant species.
One variety has a red flower colour and no scent.
The other variety has a white flower colour and a perfumed scent.
The farmer wants to produce a variety that has the red flower colour and the perfumed scent.
Explain how the farmer could achieve this.
An explanation makes reference to three of the following:
- selective breeding / artificial selection
- cross red (flower)/ unscented (flower) with white (flower) / scented (flower)/ eq
- select / breed / offspring with red and scent /eq
- repeat / for many generations eq
Ignore ref to GM as it is the farmer
Cross varieties / the plants
ignore desired characteristics alone
Read the passage below. Use the information in the passage and your own knowledge to answer the questions that follow.
Physic Gardens
The photograph shows a physic garden. Physic gardens are places where people grow many types of plants that produce medicinal drugs.

People have grown medicinal plants in physic gardens for thousands of years. It was estimated in 2016 that out of 30000 plant species that are known to be useful to humans, 17000 of these plant species have medicinal uses.
Medicinal plants produce a wide range of types of drugs including alkaloids, glycosides, terpenes and polyphenols.
Alkaloids are bitter-tasting, alkaline chemicals found in many different plant species. Alkaloids are often toxic in high doses so care needs to be taken when using them. Examples of alkaloids are caffeine and quinine. Caffeine, found in tea and coffee, can stimulate the adrenal glands. Quinine from the bark of the cinchona tree has been used for many centuries to kill the parasite that causes malaria.
An example of a glycoside drug is senna. This drug is extracted from the Senna plant and is used to treat constipation. The drug affects muscles in the intestine.
Terpenes are chemicals that often have strong smells. Terpenes are thought to have evolved to prevent herbivore animals from eating certain plants. An example of a plant that produces terpenes is thyme. The terpenes in thyme have an antiseptic effect which means they can be used to sterilise wounds.
Phytoestrogens are molecules found in plants such as angelica and have been used for many years to treat problems with human fertility. Examples of polyphenols are phytoestrogens, taxol and curcumin. Polyphenols have many roles within plants and many are used as medicines. Taxol is a drug extracted from yew trees and is used to prevent cell division in human body cell tumours.
Curcumin is a drug produced by the turmeric plant. Curcumin can help widen the airways to the lungs so can help people with asthma breathe.
Scientists are looking to reproduce many of these drugs artificially. They are developing genetically modified bacteria containing genes that will allow these drugs to be mass produced in fermenters.
Scientists are trying to insert the genes for medicinal drugs into bacteria.
Describe how named enzymes are used to place genes into plasmids to transfer the genes from plants into bacteria (lines 27 to 29 ).
A description that that makes reference to the following:
- restriction enzyme / endonuclease, AND cuts gene / DNA / plasmid
- ligase AND joins, DNA / genes / plasmid
Accept restrictive Ignore restricted
Accept lygase / lygaze Reject lipase
Micropropagation is used to produce plant clones.
The process involves growing explants in vitro.
State what is meant by the term in vitro.
in glass / in test tube / in (Petri) dish
The explants grow new roots and shoots.
A student investigates the effect of pH on the growth of new shoots.
The table shows the student's results.

Describe a procedure the student could use to obtain explants and produce these results.
A description that makes reference to five of the following points:
- use forceps / scalpel / knife / eq to remove explants / pieces from plant ( 1 )
- wash in bleach / hypochlorite / ethanol / alcohol / sterile wipes / eq
- (grow on) agar
- nutrients / minerals / carbohydrates / amino acids / named mineral / eq / growth factors / hormones
- add acid / alkali / buffer ( 1 )
- use several / multiple explants / repeat
- control temperature / carbon dioxide / light ( 1 )
- sterile agar / sterile tubes
- same age plant / same plant / same size of explants / eq
- count number of shoots after same / stated time
Allow sterile cotton wool /
Allow disinfected / ensure sterile conditions / sterilise
equipment
Give two benefits of using micropropagation to produce new plants rather than using seeds.
1
2
An answer that makes reference to two of the following points:
- lots produced
- genetically identical / no variation / all same type / identical / same characteristics / guaranteed plant / they are clones
- can grow GM plants / eq
- faster / quicker ( 1 )
- any time of year ( 1 )
- can be used for plants that are hard to germinate / grow from seed
Farmers can increase their crop yield by growing crops in a temperature controlled glasshouse.
Explain how increasing the temperature can result in an increase in crop yield.
An explanation that makes reference to two the following points:
- as temperature increases so does (rate of) photosynthesis
- increased (kinetic) energy (of molecules) / molecules move faster / more collisions
- optimum temperature for enzymes ( 1 )
- more glucose / starch / sugar / carbohydrate / eq produced
Farmers sometimes use a type of heater that burns gas or oil to raise the temperature of their glasshouse to improve crop yield.
Explain why they use this type of heater to improve crop yield.
An explanation that makes reference to two the following points:
- it releases CO2
- CO2 a reactant / used in / needed in / for photosynthesis / eq
- as CO2 is limiting factor
Some farmers also add chemical fertilisers to their crops in a glasshouse.
Different minerals are added to chemical fertilisers. Explain one mineral that should be added to these fertilisers.
An explanation that makes reference to the following points:
- nitrate
- for amino acids / proteins
- magnesium
- for chlorophyll for chloroplasts
Allow other minerals and their function
ignore nitrogen
eg phosphate for ATP / DNA
can only gain function if mineral is correct