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Edexcel IGCSE Biology (c) Biological molecules Question Bank

Practise biological molecules through food tests, enzyme investigations, data interpretation, rate calculations and explanations of denaturation.

Syllabus
First assessment 2019
Course
Biology 4BI1

Exam points

  • state elements in carbohydrates and proteins, using formula-style evidence where required
  • interpret food-test and enzyme investigation results from tables, graphs and colour changes
  • explain enzyme activity changes using active sites, optimum conditions and denaturation

(c) Biological molecules question 1

[Maximum number: 1]

Read the passage below. Use the information in the passage and your own knowledge to answer the questions that follow.

Pollination in fruit trees

Plants need to be pollinated to produce fruits and seeds. Some plants are self-pollinated and others are cross-pollinated.

Self-pollination is usually the transfer of pollen from flowers on the same tree. In fruit trees, self-pollination also includes the transfer of pollen from another tree of the same cultivar. A cultivar is a genetically identical group of trees produced by selective breeding. Self-pollinating plants need bees or other insects to transfer pollen. Examples of self-pollinated fruit trees are plum, apricot and peach that have sweet-tasting, soft fruits.

Figure for Question (c) Biological molecules question 1 — Edexcel IGCSE Biology
Figure for Question (c) Biological molecules question 1 — Edexcel IGCSE Biology

Other fruit trees require cross-pollination and can only produce fruit by fertilisation from a different cultivar. Cross-pollination is the transfer of pollen from one cultivar to the flower of a different cultivar. Self-incompatibility prevents the same plant or cultivar from fertilising its own flowers. For example, the pollen from one cultivar of an apple tree will not fertilise trees of the same cultivar. Most hard fruit trees such as pear and apple require the presence of two different compatible cultivars for pollination to result in fruit production.

Pollen can be transferred by wind, insects or birds. Many fruit trees are pollinated by insects. Nut trees are usually pollinated by wind.

After pollination the pollen grain must germinate to lead to fertilisation. The success of pollination and fertilisation depends on favourable environmental conditions. It also requires the right pollen grain reaching the right flower, as pollen can only fertilise specific, compatible flowers. For example, pollen from a peach flower will not pollinate apple flowers.

Cross-pollinating fruit trees should be planted no more than 60 m apart. This will ensure that bees visit the trees often enough to ensure pollination. Nut trees need to be planted no more than 15 m apart.

A further complication is that a few apple and pear cultivars, known as triploids, produce sterile pollen. Triploid cultivars have three sets of chromosomes within their cells. A triploid cultivar will require another cultivar for pollination, and the trees must flower at the same time.

Fruit trees often produce sweet-tasting fruit containing sugars.

Sugars are carbohydrates.

Name the three elements in carbohydrates.

(c) Biological molecules question 2

[Maximum number: 3]

Wheat seeds contain stores of a large insoluble molecule.
This molecule is digested by amylase as the seeds germinate.

Question (a)

(a)

What is the name of this large insoluble molecule?

A

glucose

B

lipid

C

protein

D

starch

[ 1 ]

Question (b)

(b)

The graph shows the results of the student's investigation.

Figure for Question (b) — Edexcel IGCSE Biology
[ 2 ]

Question (i)

(i)

Suggest why the rate of oxygen absorption is greater at 22C22^{\circ} \mathrm{C} than at 12C12^{\circ} \mathrm{C}.

[ 2 ]

(c) Biological molecules question 3

[Maximum number: 5]

Pineapple juice contains a protease called bromelain.
A student uses this method to investigate the digestion of solid gelatine protein by bromelain.
- place solid gelatine protein into a test tube up to a height of 5 cm
- mix 5 cm35 \mathrm{~cm}^{3} pineapple juice with 1 cm31 \mathrm{~cm}^{3} of pH 4 buffer
- place 1 cm31 \mathrm{~cm}^{3} of the pineapple juice and buffer solution on top of the gelatine
- leave for one hour in a water bath set to 37C37^{\circ} \mathrm{C}
- measure the height of the solid gelatine and use it to calculate the volume of gelatine that has been digested

Repeat the method three more times.
The diagram shows part of the student's method.

Figure for Question (c) Biological molecules question 3 — Edexcel IGCSE Biology

Question (a)

(a)

The student repeats the investigation with different pH buffers.

The table shows their results.

Table for Question (a) — Edexcel IGCSE Biology
[ 3 ]

Question (i)

(i)

Explain the effect of changing the pH on the mean volume of gelatine digested.

[ 3 ]

Question (b)

(b)

Describe how to test for the presence of protein.

[ 2 ]
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