8. Transport in plants

Syllabus
0610–2026–2027
Section
8
Level
—

8.1 Xylem and phloem

Syllabus
0610–2026–2027
Topic
8.1
Level
—

Compare the functions of xylem and phloem

Xylem and phloem are the two transport tissues in a plant vascular bundle, but they carry different substances and xylem also has a supporting role.

Tissue Substances transported Additional function
xylem water and mineral ions supports the plant
phloem sucrose and amino acids —

Water and dissolved mineral ions taken up by roots travel in xylem. Sucrose made by photosynthesis and amino acids are transported in phloem to parts of the plant that use or store them.

Xylem does not transport sucrose, and phloem does not carry the plant's main water stream. Starch is insoluble and is not the transport sugar; sucrose is.

Identify xylem and phloem in roots, stems and leaves

In a non-woody dicotyledonous plant, xylem and phloem occur together as transport tissues, but their pattern changes between root, stem and leaf sections.

Organ section Xylem position Phloem position
root central star- or cross-shaped region groups between the arms of the xylem
stem inner side of each vascular bundle; bundles form a ring outer side of each vascular bundle
leaf upper side of a vascular bundle in a vein lower side of the bundle

First identify the organ from its overall tissue pattern. Then locate each vascular bundle and use the inside–outside or upper–lower relationship to distinguish xylem from phloem.

Do not use one position rule for every organ: central xylem identifies a dicot root, whereas a ring of separate bundles identifies a non-woody dicot stem.

Relate xylem vessel structure to function

A xylem vessel is a long, hollow tube specialised to carry water and mineral ions while helping support the plant.

Structural feature Functional consequence
thick walls containing lignin strengthen the vessel, support the plant and resist collapse
no cell contents leaves a hollow lumen with little resistance to water flow
cells joined end to end with no cross walls forms one long continuous tube so water can flow without end-wall barriers

lignified continuous hollow vessel → strong unobstructed pathway → transport of water and mineral ions plus structural support

This syllabus objective is limited to lignified thick walls, absence of cell contents and absence of cross walls. Do not confuse a xylem vessel with a living phloem transport cell.

8.2 Water uptake

Syllabus
0610–2026–2027
Topic
8.2
Level
—

Identify root hair cells and their functions

A root hair cell is an epidermal cell near the tip of a young root. Its long, thin projection extends between soil particles.

Substance taken up Root hair cell function
water absorbs water from the soil
mineral ions absorbs dissolved mineral ions from the soil

In a diagram, look for one cell on the outside of a root with a single hair-like extension. The projection is part of the cell, not a separate root.

Root hairs absorb water and mineral ions; they do not absorb glucose from soil and they are not the branching whole roots visible to the naked eye.

Explain why root hairs have a large surface area

Each root hair is a long extension of a root epidermal cell. Many root hairs together create a very large contact area with the thin films of water around soil particles.

many long root hairs → larger surface area in contact with soil → more membrane available for uptake at the same time → increased uptake of water and mineral ions

A root system with many root hairs can explore more soil-water contact than a smooth root surface of the same size, increasing the rate and total opportunity for uptake.

Large surface area increases uptake; it does not mean each root hair contains a larger volume of cell sap.

Trace water from soil through root, stem and leaf

The required pathway is: soil water → root hair cells → root cortex cells → xylem → mesophyll cells in the leaf.

Stage Where the water is
1 enters root hair cells at the root surface
2 crosses root cortex cells toward the centre
3 enters xylem and travels through root and stem to the leaf
4 leaves xylem and enters mesophyll cells

The sequence connects uptake at the root surface to delivery inside the leaf. Xylem is the long-distance transport stage between the root cortex and leaf mesophyll.

Do not place xylem before the root cortex: water must cross from root hair cells through cortex cells before entering the central xylem.

Investigate the pathway of water using a stain

A coloured, water-soluble stain can reveal which above-ground plant tissues carry water.

Step Action and purpose
1 place the cut base of a leafy shoot or celery stalk in coloured water
2 leave it long enough for the colour to move up the shoot
3 cut thin transverse sections of the stem and examine leaf veins
4 record the positions that are stained and compare them with vascular-bundle structure

The stain appears in the xylem of the stem and leaf veins. This shows that water moves through the above-ground parts of the plant in xylem.

The stain marks the water-conducting xylem, not every tissue in the section. Use a freshly cut surface and make thin sections carefully so the stained positions can be identified.

8.3 Transpiration

Syllabus
0610–2026–2027
Topic
8.3
Level
—

Define transpiration

Transpiration is the loss of water vapour from the leaves of a plant.

Water reaches a leaf as liquid in the xylem but leaves it as water vapour. The loss occurs mainly through stomata in the leaf surface.

Transpiration names the overall loss of water vapour from leaves. It is not the upward movement of liquid water in xylem, and it is not translocation of sucrose in phloem.

Trace water vapour out of a leaf

Water evaporates from the moist surfaces of mesophyll cells into the interconnecting air spaces inside the leaf. The water vapour then diffuses out through the stomata.

Stage State and location of water Process
1 liquid water on mesophyll-cell surfaces evaporation
2 water vapour in leaf air spaces diffusion toward the outside air
3 water vapour passing through stomata diffusion out of the leaf

Diffusion occurs because the concentration of water vapour is usually higher inside the leaf air spaces than in the surrounding air.

Evaporation is the liquid-to-vapour change at mesophyll surfaces; diffusion is the later net movement of vapour through the air spaces and stomata.

Investigate temperature and wind effects on transpiration

Change temperature or wind speed one factor at a time and measure transpiration rate while keeping the other conditions constant.

Investigation choice What to do
response measure record plant mass loss per unit time, or use bubble movement/water uptake in a potometer as a proxy
temperature compare several controlled temperatures while wind speed stays constant
wind speed compare controlled air speeds or fan distances while temperature stays constant
controls same species, leaf area, duration, light intensity and humidity
reliability repeat each condition and calculate a mean rate

Increasing temperature increases transpiration rate. Increasing wind speed also increases transpiration rate. Plot the measured rate against the changed factor and describe the pattern using the data.

A potometer measures water uptake, not water loss directly. Water uptake is used as an estimate of transpiration only when the apparatus is airtight and other water uses are recognised as a limitation.

Link leaf structure to water-vapour loss

A leaf presents a large moist internal surface to air and provides stomatal openings through which that air connects to the atmosphere.

Leaf feature Effect on water-vapour loss
many interconnecting spaces between mesophyll cells expose a large internal surface and provide a route for vapour movement
many stomata provide a larger total area of exit pores
larger stomatal openings provide a larger total diffusion area to the outside air

Together, the large mesophyll surface and stomatal exit area allow more water to evaporate and diffuse out at the same time, increasing water-vapour loss.

The air spaces do not make water themselves. They expose moist mesophyll surfaces and connect them to stomata, where vapour leaves the leaf.

Explain transpiration pull in xylem

Water loss from the leaf creates a pulling force at the top of the xylem called transpiration pull.

The pull draws a column of water molecules upwards through the xylem. Forces of attraction between water molecules hold the molecules together, so the pull is transmitted down the continuous column.

water vapour lost from leaves → transpiration pull develops → continuous water column is pulled upward → attraction between water molecules keeps the column together

Water is not pushed up the xylem by active transport. The syllabus mechanism is a pull from transpiration acting on a cohesive column of water.

Explain how temperature, wind and humidity affect transpiration

Environmental factors change evaporation from mesophyll surfaces or the water-vapour concentration gradient between the leaf and the surrounding air.

Change Effect on rate Why
higher temperature increases water molecules have more kinetic energy, so evaporation and diffusion are faster
higher wind speed increases moving air removes the humid layer near the leaf, maintaining a steep concentration gradient
higher humidity decreases outside air already contains more water vapour, so the concentration gradient is smaller

The highest rate is expected in warm, windy, low-humidity air; the lowest in cool, still, high-humidity air, provided stomata and water supply do not become limiting.

High humidity does not speed evaporation. It reduces the water-vapour gradient from leaf to air, so net diffusion and transpiration slow down.

Explain how and why plants wilt

Wilting occurs when water loss by transpiration is faster than water uptake by the roots.

Plant cells lose water, their vacuoles shrink and turgor pressure falls. The cells become flaccid and no longer press firmly against one another, so leaves and non-woody stems droop.

water loss exceeds uptake → cells lose water → turgor pressure falls → cells become flaccid → leaves and stems wilt

A wilted plant has lost turgor; its cells are flaccid, not turgid. Wilting is caused by an imbalance between water loss and uptake, not simply by high temperature alone.

8.4 Translocation

Syllabus
0610–2026–2027
Topic
8.4
Level
—

Define translocation in phloem

Translocation is the movement of sucrose and amino acids in phloem from sources to sinks.

Part of the definition Required idea
substances sucrose and amino acids
tissue phloem
direction from a source to a sink

Movement may be upwards or downwards in the plant because the location of a source relative to a sink can differ.

Translocation is not transpiration. Phloem carries sucrose and amino acids from sources to sinks; xylem carries water and mineral ions.

Distinguish sources from sinks

A source releases sucrose or amino acids into phloem; a sink removes them from phloem to use or store them.

Role What it does Common examples
source releases transported nutrients mature photosynthesising leaves; a storage organ releasing reserves
sink uses or stores transported nutrients growing shoot or root tips, flowers, fruits, seeds and storage organs filling with reserves

Phloem therefore links producing or releasing regions to regions of growth, respiration, reproduction or storage.

A source is defined by release and a sink by use or storage. The terms do not mean 'top' and 'bottom' of the plant, so movement is not always downward.

Explain why organs can switch between source and sink

Source and sink are changing roles, not permanent labels for particular organs.

Organ or stage Role Reason
young leaf or growing shoot sink needs imported sucrose and amino acids for growth
mature photosynthesising leaf source produces and releases sugars
storage organ while reserves accumulate sink receives and stores transported nutrients
storage organ during sprouting or flowering source releases reserves to support new growth

For example, a potato tuber can be a source in spring while a new shoot grows, then become a sink later when mature leaves send sucrose back for storage.

An organ changes role when its balance of production or reserve release versus use or storage changes; its physical position does not determine the role.