IB Biology HL 2.4.4 Osmosis experiment design and data Question Bank
Practise IB Biology HL 2.4.4 by designing and evaluating experiments that estimate solute concentration in plant cells.
- Syllabus
- First assessment 2025
- Course
- Biology HL
- Level
- HL
Practise IB Biology HL 2.4.4 by designing and evaluating experiments that estimate solute concentration in plant cells.
Data was collected on rabbit red blood cells that were exposed to sodium chloride (NaCl) and scorpion venom. Under some osmotic conditions red blood cells swell and burst, releasing hemoglobin (hemolysis). The graph shows the response of red blood cells to different concentrations of sodium chloride, with and without scorpion venom.

Describe how the variables would be controlled in an experiment to estimate the osmolarity of plant tissue.
a. weigh all pieces to ensure same amount of plant material/allow comparisons
b. control surface area by having same shape/ size
c. carry out experiment at same temperature e.g. using a water bath or constant room temperature
d. all samples in solution for same length of time
e. all samples from same plant (to minimize variability)
f. use a range of solutions of the same solute
g. constant method of removing excess fluid before weigh tissue samples
Marking guidance:
Do not accept just a list of controlled variables.