A3.1.5—Difficulties in distinguishing species
Distinguishing species can be difficult when populations diverge gradually, hybridize, or show genetic evidence that does not match simple ranges.
- Syllabus
- First assessment 2025
- Objective
- A3.1.5
- Level
- HL
Distinguishing species can be difficult when populations diverge gradually, hybridize, or show genetic evidence that does not match simple ranges.

Coverage 2015–2015 · Updated 15 Jul 2026
The biological species concept defines a species as a group of organisms that can breed with one another and produce fertile offspring.
Successful reproduction allows gene flow within the species, whereas reproductive isolation limits gene flow between species. During speciation, separated populations usually diverge gradually rather than becoming different species in one step.
To apply the concept: identify whether breeding is possible, determine whether offspring are viable and fertile, and distinguish actual reproductive isolation from populations merely being unable to meet. Because divergence is continuous, the point at which two populations receive different species names can be partly arbitrary.
Two geographically isolated populations may never meet in nature but could still produce fertile offspring if brought together. Their separation alone therefore does not prove that they are different biological species.
The concept is difficult to test for fossils, asexual organisms and geographically isolated populations, and competing species definitions may give a different boundary.
This objective is assessed through data analysis, commonly using State.
State
Build the answer around this relationship: Speciation is gradual, so boundaries between diverging populations can be uncertain.
Representative question
State with a reason whether the genetic evidence shows that the western coyote and the grey wolf have overlapping ranges.
no overlapping ranges since no haplotypes/C9, C19 from western coyotes present with haplotype/C22 from grey wolf in any of the hybrids
For SL, A3.1 is really one evidence map. Variation explains why individuals differ. Morphology and binomial nomenclature help group and name organisms. The biological species concept uses interbreeding and fertile offspring, but boundaries can be difficult during gradual speciation. Chromosomes and karyograms add cellular evidence. Genomes, SNPs, genome size, and whole genome sequencing add molecular evidence. The skill is choosing the right evidence for the question.