CAIE A-Level Biology A2 12.2.13 Redox Indicator Investigation Questions
Explain redox indicator investigations in respiration using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- A2
Explain redox indicator investigations in respiration using evidence from each question and mark scheme.
Describe how you would carry out an investigation to calculate the effect of substrate concentration on the rate of respiration of yeast in anaerobic conditions using a redox indicator, such as DCPIP or methylene blue.
any eight from:
1 vary / different / range of, glucose / fructose, concentrations ;
2 control / same, volume of yeast (suspension) ;
3 control / same, volume of glucose (solution) ;
4 control / same, volume / concentration, of indicator (solution) ;
5 control temperature (between 20−45∘C ) ;
6 oil layer to exclude, air / oxygen ;
7 indicator turns from blue (start / oxidised) to colourless (end / reduced) ;
8 due to, hydrogen atoms / H+/ electrons, from, glycolysis / dehydrogenation / respiration ;
9 time how long it takes for colour change OR fix time interval and measure with colorimeter ;
10 repeat experiment ;
11 calculate mean(s) ;
12 graph with glucose concentration (on x axis) and, time taken / \% absorbance / \% transmission / rate (on y ) ;
13 rate is 1 / t;
14 AVP ;