CAIE A-Level Biology A2 12.2.13 Redox Indicator Investigation Questions
Explain redox indicator investigations in respiration using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- A2
Explain redox indicator investigations in respiration using evidence from each question and mark scheme.
The activity of succinate dehydrogenase, an enzyme in the Krebs cycle, is used as a measure of the rate of respiration in the mitochondria. The redox reaction catalysed by succinate dehydrogenase is shown in Fig. 8.2.
Fig. 8.2
The activity of succinate dehydrogenase can be measured using a redox indicator.
Name a redox indicator and explain why this indicator can be used to measure the activity of succinate dehydrogenase.
DCPIP / methylene blue ;
changes, colour / blue to colourless, when, reduced / accepts electrons (from succinate) ;