IB Chemistry SL 2.2.10 Chromatography

Practise interpreting chromatograms through competing attractions to mobile and stationary phases. Calculate retardation factors from measured distances, compare component…

Exam points

calculate Rf by dividing a spot's distance by the solvent-front distance from the baseline
link greater movement to the balance of mobile- and stationary-phase attractions
justify a level baseline, solvent below the spots and a sealed chromatography chamber

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Proteins are made of long chains of amino acids.

A mixture of amino acids was spotted onto chromatography paper and eluted with a solvent mixture. The following spots were seen after the paper had been developed with ninhydrin.

Figure for Question 2.2.10—Chromatography question 1 — IB Chemistry SL

Determine the $R_{\mathrm{f}}$ value of the amino acid marked as X .

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2.2.10—Chromatography question 1