Unity and Diversity connects water, molecules, cells, classification, evolution and biodiversity into evidence-based explanations of shared life patterns and biological variation.
Syllabus
First assessment 2025
Section
—
Level
SL
Exam analysis
Published Concept evidence in Unity and Diversity repeatedly links cell structures, nucleic acids, organism diversity and evolution. The strongest pattern is not isolated recall, but explaining how molecular and cellular evidence supports biological unity, variation and classification.
Water’s polarity and hydrogen bonding create solvent, thermal, cohesive and adhesive properties that support metabolism, transport, cooling and aquatic life.
Syllabus
First assessment 2025
Topic
A1.1
Level
SL
Life runs in a shared liquid environment
The earliest cells arose in water, where the surrounding ocean reduced exposure to ultraviolet radiation. Modern cells still contain a high proportion of water, and most cellular chemistry occurs in an aqueous phase.
As a biological medium, water allows:
dissolved reactants to diffuse and encounter one another
enzymes and substrates to interact in the same liquid phase
solutes to move through cytoplasm, blood, tissue fluid and plant transport systems
A medium provides the environment for a process. Water is a reactant only in reactions that chemically consume it. Its value as a medium depends on the molecular interactions developed next.
Molecular shape turns bond polarity into a polar molecule
Oxygen attracts the shared electrons in each O–H covalent bond more strongly than hydrogen. Oxygen therefore carries a partial negative charge, δ−, while each hydrogen carries δ+. Because the molecule is bent, the two bond polarities do not cancel.
A hydrogen bond is the attraction between a δ+ hydrogen on one water molecule and a δ− oxygen on another. It is an intermolecular attraction, not the covalent bond holding O and H together inside one molecule.
One hydrogen bond is weak compared with an O–H covalent bond. Water's unusual properties arise because enormous numbers of hydrogen bonds act together while continually breaking and reforming.
Cohesion has a bulk effect and a surface effect
Cohesion is attraction between water molecules. Many hydrogen bonds together allow a body of water to transmit force without the molecules separating easily.
In xylem, evaporation from leaves creates tension. Cohesion keeps water molecules connected, so the pull is transmitted down a continuous column and the column resists breaking.
At the surface, water molecules have no neighbours above them, so cohesive attractions have a net inward effect. The surface resists stretching or breaking; a small pond skater with water-repellent legs can spread its weight without rupturing it.
Cohesion maintains the xylem column but does not create the transpiration pull. Surface tension supports an organism at a boundary; buoyancy supports an immersed body through displaced fluid.
Adhesion depends on what water is touching
Force
Attractive contact
Main biological consequence
Cohesion
water ↔ water
maintains a connected xylem column that can resist tension
Adhesion
water ↔ polar or charged surface
wets cellulose and pulls water along narrow spaces
In narrow soil pores and spaces between cellulose fibres, adhesion pulls water along the hydrophilic wall. Cohesion draws neighbouring water molecules with it, producing capillary movement and keeping leaf cell walls moist.
Adhesion helps water enter and wet narrow spaces, but cohesion is the dominant force preventing a transpiring xylem column from breaking under tension.
Partial charges predict what water can carry
Around Na+, the δ− oxygen ends of water face inward; around Cl−, the δ+ hydrogen ends face inward. Water–ion attractions separate the ions and stabilize a mobile hydration shell around each one.
Substance
Interaction with water
Biological result
ions and polar molecules
charges or polar groups attract water; polar groups may hydrogen-bond
dissolution enables diffusion, reactions and transport
non-polar molecules or regions
no equivalent favourable attraction
poor solubility helps form water-excluding boundaries such as membrane interiors
O₂ and N₂ are non-polar and only slightly soluble. Respiratory pigments such as haemoglobin therefore raise the oxygen-carrying capacity of blood far above that of water alone.
Water changes the costs of temperature control and movement
Property of water compared with air
Opportunity
Constraint
higher specific heat capacity
habitat and water-rich body temperatures change slowly
much energy is exchanged before water temperature changes
higher thermal conductivity
heat moves readily through the surrounding water
a warm animal loses heat faster than in air
much greater density and buoyancy
displaced water supports body mass
upthrust must be overcome during a dive
greater viscosity
feet and flippers can push against the fluid
drag raises the cost of movement
Specific heat capacity describes the energy needed to change temperature; thermal conductivity describes how readily heat passes through a material. Water can therefore provide a stable habitat while still removing heat quickly from a warm animal.
Predict the adaptation from the constraint: insulation limits heat transfer, streamlining reduces drag, broad propulsive surfaces push water backward, and increased mass or reduced trapped air helps an animal dive.
A loon and a seal meet the same habitat in different ways
Aquatic challenge
Black-throated loon
Ringed seal
reduce heat loss
trapped air in feathers limits conduction and convection
thick blubber insulates beneath the skin
produce thrust with low drag
streamlined body; rear webbed feet push water backward
streamlined body; flippers provide propulsion
manage buoyancy while diving
solid bones and compressed air reduce upthrust
blubber adds buoyancy, so swimming force is needed to descend
An adaptation is explained by linking a structural feature to the relevant property of water and then to its consequence for heat balance, propulsion or diving—not by listing the feature alone.
Recover the chain from molecule to biological consequence
Unequal electron sharing + bent geometry → a polar molecule → hydrogen bonding and attraction to charged or polar matter. Begin every explanation from the interaction that produces the property.
Interaction or physical property
Biological opportunity
Constraint or boundary
water–water attraction
xylem continuity; surface tension
cohesion does not create transpiration pull
water–surface attraction
capillary movement and wet cell walls
adhesion is not the main force resisting xylem tension
attraction to ions and polar solutes
reactions, diffusion and transport
non-polar O₂ and N₂ remain poorly soluble
heat capacity, conductivity, buoyancy and viscosity
stable habitat, support and propulsion
heat loss, diving cost and drag require adaptations
Connect cohesion to a biological consequence
[ 1 ]
Water shows strong cohesive properties. Which of the following can occur because of the cohesive properties of water?
Recognise adhesion from the contact pair
[ 1 ]
Which of the following is a consequence of the adhesive properties of water?
Explain a hydrogen bond precisely
[ 3 ]
Outline how hydrogen bonds form in water.
Use solvent behaviour to explain transport
[ 1 ]
Which can be explained by the solvent properties of water?
Make a comparison, not two isolated descriptions
[ 2 ]
Distinguish between the physical properties of water and air.
A1.2 Nucleic acids
Explore how nucleotide components, bonding, base pairing and sequence give DNA and RNA their structures, information capacity, shared code and experimental significance.
Syllabus
First assessment 2025
Topic
A1.2
Level
SL
DNA stores the inherited information of living organisms
In every living organism, DNA is the genetic material. Its base sequence stores information that can be copied before cell division and passed between generations.
In eukaryotic cells, most DNA is organized into chromosomes in the nucleus. Mitochondria and chloroplasts also contain smaller DNA genomes.
Some viruses use RNA as their genetic material. This does not contradict the rule for living organisms because viruses depend on host cells and are not classified as living.
A nucleotide has three covalently connected parts
Every nucleotide contains:
a phosphate group
a five-carbon pentose sugar
a nitrogenous base
The sugar is the central connector: the base and phosphate are both bonded to it. DNA nucleotides contain deoxyribose; RNA nucleotides contain ribose. A base alone is not a nucleotide.
Family
Bases
Ring structure
Purines
adenine (A), guanine (G)
two rings
Pyrimidines
cytosine (C), thymine (T), uracil (U)
one ring
Condensation links nucleotides into a strong backbone
A condensation reaction joins the phosphate of one nucleotide to the sugar of the next, releases water and forms a covalent phosphodiester bond. Repetition produces a long polynucleotide.
Alternating sugar and phosphate form a continuous, unvarying backbone. The bases project from the sugars, so the molecule can keep the same structural support while its base sequence changes.
The changing base sequence carries the message
DNA uses: A, T, C and G
RNA uses: A, U, C and G
The sugar–phosphate backbone repeats, so it cannot encode a changing message. The order of bases can vary. When an RNA sequence is read in groups of three, each codon specifies an amino acid or a control signal, connecting nucleotide order to polypeptide sequence.
Four bases produce 64 possible triplets: 4 × 4 × 4. Different codons can specify the same amino acid, so the code is redundant.
One RNA polymer supports three different roles
RNA is usually a relatively short, single-stranded polynucleotide made by condensation of ribonucleotides. It contains ribose and the bases A, U, C and G.
RNA
Distinct contribution to protein synthesis
mRNA
carries the codon sequence copied from DNA to a ribosome
tRNA
pairs its anticodon with an mRNA codon and delivers the corresponding amino acid
rRNA
forms the structural and catalytic core of the ribosome
These molecules share the same type of backbone and bases, but differences in sequence, length and folding give them different functions.
Complementary pairs connect the two DNA strands
DNA contains two polynucleotide strands arranged antiparallel and wound into a double helix. The sugar–phosphate backbones face outward; bases project inward and pair through hydrogen bonds.
Adenine pairs with thymine: A–T
Cytosine pairs with guanine: C–G
In a structural diagram, attach every base to a sugar, alternate sugar and phosphate along each backbone, and show the two strands running in opposite directions. The helical twist is not required.
DNA and RNA differ in sugar, base and strand arrangement
Feature
DNA
RNA
Pentose sugar
deoxyribose
ribose
Distinctive base
thymine (T)
uracil (U)
Usual strand arrangement
two antiparallel strands
one strand
Typical relative length
very long
shorter
Both are polynucleotides with a sugar–phosphate backbone and bases A, C and G. Identify the differences using matched features rather than treating them as unrelated molecules.
Complementarity lets one strand specify another
Because each base has one complementary partner, the sequence of a template strand determines the sequence that is assembled against it. This is the common molecular logic behind copying and expression.
Process
Pairing used
Product specified by the template
DNA replication
A–T and C–G
a new DNA strand
Transcription
A–U and C–G
an RNA strand
Translation
mRNA codon–tRNA anticodon
the order of amino acids
Example: a DNA template written 3′-A C G T-5′ produces RNA 5′-U G C A-3′. The strands are complementary and antiparallel, not identical.
Four bases generate an immense sequence space
4n
For a sequence of n positions, each position can contain one of four bases, giving 4ⁿ possible sequences. Changing the length or the order changes the information, so long DNA molecules have enormous storage capacity.
Genome size is the total amount of DNA, whereas gene number counts genes. Neither value alone is a simple measure of organism complexity.
A nearly universal genetic code points to common ancestry
Across almost all living organisms, the same codons specify the same amino acids. Genes involved in transcription, translation and ribosome function are also highly conserved.
A complex decoding system shared so widely is best explained by inheritance from a common ancestor. Later evolution changed DNA sequences, but retained the core code and molecular machinery.
The code is nearly, not absolutely, universal. Minor exceptions do not erase the much larger conserved pattern. Redundancy also means that some base substitutions are synonymous and do not change the amino acid.
From nucleotide structure to inherited information
Build and store
Phosphate + pentose sugar + base form a nucleotide.
Condensation creates a covalent sugar–phosphate backbone.
The backbone repeats while base order varies, so the polymer stores information.
Copy and express
Complementary pairing lets one strand specify another. DNA replication preserves information; transcription produces RNA; codon–anticodon pairing helps translate the message into an amino-acid sequence.
Identify from
DNA
RNA
Sugar
deoxyribose
ribose
Distinctive base
T
U
Usual structure
double-stranded
single-stranded
The same structure-to-expression system operates across life, and the nearly universal code is evidence that living organisms inherited it from a common ancestor.
DNA as genetic material
[ 1 ]
The Human Genome Project completed the sequencing of the human genome by the year 2003. Which could have been a source of the entire genome in humans?
Components of a nucleotide
[ 3 ]
Draw a labelled diagram to show the structure of a DNA nucleotide.
Drawing canvas
Sugar-phosphate bonding
[ 2 ]
Outline the bonding between DNA nucleotides.
RNA as a polymer
[ 4 ]
Draw labelled diagrams to show the structure of RNA nucleotides and how they are linked together to form a molecule of RNA.
Drawing canvas
DNA as a double helix
[ 6 ]
Draw a simple labeled diagram to show the structure of a double stranded DNA molecule, comprising four nucleotides.
Drawing canvas
Differences between DNA and RNA
[ 3 ]
Distinguish between the structures of DNA and RNA.
Complementary base pairing
[ 1 ]
Deduce the base indicated by X on the diagram.
Diversity of DNA base sequences
[ 4 ]
Explain the diversity of possible base sequences in nucleic acids.
Conservation of genetic code
[ 1 ]
Some yeast genes can be replaced by human genes that then continue to produce the same human proteins in the yeast cells. Which statement helps to explain this evidence?
A2.2 Cell structure
Cell structure connects microscopy, universal cellular organization, prokaryotic and eukaryotic diversity, specialized exceptions, differentiation, and evolutionary explanations for complex cells and multicellularity.
Syllabus
First assessment 2025
Topic
A2.2
Level
SL
A cell is an integrated living unit
A cell is the smallest membrane-bounded unit that can carry out the processes of life. A tissue is different: it is a group of cells cooperating in a shared function.
Cell theory states that:
living organisms consist of one or more cells
cells are the basic units of structure and function
new cells arise from pre-existing cells
The theory is a powerful biological model, not a claim that every cell has the same appearance. Later examples such as multinucleate fibres and mature cells without nuclei test its boundaries without removing the central idea that living organization is cellular.
Prepare and focus a specimen without losing it
1
Make a thin temporary mount, add a coverslip, and use an appropriate stain when transparent structures need contrast.
2
Start with the low-power objective. Centre the specimen while the field of view is wide.
3
Bring the image into focus with coarse adjustment, then sharpen it with fine adjustment while moving the stage away from the objective.
4
Rotate to a higher-power objective only after centring. Use fine focus only because the working distance is now small.
5
Record the specimen, stain, objective and any measurement. Preparation and staining may alter appearance, so observed features must be interpreted as evidence rather than treated as untouched reality.
Calibrate before converting divisions into micrometres
M=AIA=MII=A×M
At one objective, 10 graticule divisions correspond to 20 µm, so each division represents 2 µm. A cell spanning 18 divisions is therefore 36 µm long. If its printed image is 54 mm long, convert 54 mm to 54,000 µm before calculating: M=54,000/36=1,500×.
Calibration belongs to a particular objective lens. Changing the objective changes the real distance represented by one graticule division, so the calibration must be repeated.
Choose the microscope for the evidence you need
Magnification makes an image larger; resolution allows two close points to be seen as separate. Enlarging beyond the resolution limit only produces a larger blur.
Evidence needed
Useful method
Important limit
living cells and larger structures
compound light microscopy
about 0.2 µm resolution
location of a named molecule
fluorescence or immunofluorescence
the dye or antibody identifies only its target
internal ultrastructure in a thin section
TEM
specimen is fixed, stained and viewed in vacuum
surface form
SEM
shows surface, not a thin internal section
near-native molecular structure
cryo-EM
frozen samples and computational reconstruction are required
Preparation can introduce artefacts. Confidence increases when different preparation methods reveal a consistent structure, rather than when one impressive image is accepted alone.
Four components make every cell a workable system
All cells contain:
a plasma membrane that controls exchange with the surroundings
cytoplasm in which metabolic reactions occur
DNA that stores inherited information
ribosomes that translate information into polypeptides
None of these components alone is a cell. Together they create a bounded chemical system that can maintain itself, use information and make the proteins needed for its activities.
A prokaryote concentrates life in one small compartment
A prokaryotic cell has no nucleus and no membrane-bound organelles. Its single circular, naked chromosome occupies a nucleoid region in the cytoplasm, where 70S ribosomes make proteins. In eubacteria, a peptidoglycan wall lies outside the plasma membrane.
Plasmids, capsules, pili, flagella and specialized membrane regions occur in some bacteria, not all. E. coli, Bacillus and Staphylococcus also differ in shape and wall organization, so no optional feature should be treated as the definition of a prokaryote.
Eukaryotic compartments divide and connect cellular work
A eukaryotic cell encloses linear chromosomes in a nucleus and uses membrane-bound organelles to maintain different reaction conditions. Mitochondria transfer energy to ATP; the ER, Golgi apparatus, vesicles and lysosomes form a connected system for synthesis, transport, modification and digestion.
For a secreted protein, information and material move through a coordinated route: nuclear gene → transcription → rough-ER ribosome → ER lumen → transport vesicle → Golgi modification and sorting → secretory vesicle → plasma membrane.
The cytoskeleton gives internal organization and shape. Microtubules guide vesicles and move chromosomes; actin microfilaments resist tension and contribute to cell movement and shape change.
One cell must complete every life process
A unicellular organism cannot delegate essential work to tissues. Nutrition, metabolism, homeostasis, excretion, response, movement, growth and reproduction must all be achieved within one cell.
Life process
Amoeba or Paramecium
Chlamydomonas
E. coli
nutrition
engulfs particles into food vacuoles
photosynthesizes and absorbs ions
absorbs small molecules
water balance
contractile vacuoles expel excess water
membrane transport and vacuoles regulate contents
membrane transport maintains cytoplasm
movement or response
pseudopodia or cilia respond to local conditions
flagella and light-sensitive responses
receptors and flagella in motile strains
reproduction
cell division
cell division
binary fission
The structures differ, but the same functional problem remains: matter, information and energy must be coordinated inside one bounded cell.
Plant, fungal and animal cells modify a shared eukaryotic plan
Feature
Plant cell
Fungal cell
Animal cell
wall
cellulose
chitin
absent
plastids
chloroplasts in photosynthetic cells
absent
absent
vacuoles
often one large permanent vacuole
vacuoles present
usually small and temporary
carbohydrate store
starch
glycogen
glycogen
frequent additional structures
plasmodesmata
branching hyphae in many species
centrioles; cilia or flagella in some cells
Classification should use a combination of structures. A wall alone does not prove that a cell is plant, and the absence of a chloroplast does not exclude plant tissue because many plant cells are non-photosynthetic.
Atypical cells expose where the simple model breaks
Aseptate fungal hyphae have many nuclei in continuous cytoplasm without complete cross-walls.
Striated muscle fibres form by cell fusion and remain long and multinucleate.
Mature red blood cells lose the nucleus and most organelles, gaining space and flexibility for oxygen transport but losing the ability to divide.
Sieve-tube elements lose the nucleus and depend metabolically on adjacent companion cells.
These cases do not show that cell theory is useless. They show that specialization can redistribute cellular functions across shared cytoplasm or cooperating cells, so “one small compartment with one nucleus” is not the theory itself.
Identify a micrograph by converging clues
Build an identification from several clues:
establish the imaging method and scale
locate boundaries and decide whether they are walls or membranes
look for an internal pattern, not just a familiar outline
use tissue context and neighbouring structures
state the observation before naming the structure
Here, several parallel flattened sacs plus small vesicles at their margins support Golgi apparatus. One circular membrane profile would be weak evidence because a section can cut many organelles into circles.
Absence is weaker than presence: a thin section may miss an organelle that exists elsewhere in the cell. Identification should therefore rest on visible positive evidence whenever possible.
Translate a micrograph into an evidence-based drawing
1
Trace the main boundaries and proportions from the micrograph before naming anything.
2
Use a large drawing with single clear continuous lines. Do not shade, colour or invent structures that are not visible.
3
Add ruled, non-crossing label lines that touch the named feature. A label identifies; an annotation adds a justified structure–function statement.
4
Include magnification or a calculated scale bar, with image and actual lengths converted to the same units.
Before adding an annotation, ask whether the feature can be pointed to in the drawing and whether the claimed function follows from that observed structure. Biological knowledge may guide interpretation, but it must not fabricate visual evidence.
SL synthesis: build a cell claim from evidence
Question
Evidence to use
Claim it can support
Is this a cell?
membrane-bounded cytoplasm with genetic material and ribosomes
an integrated cellular unit
How large is it?
calibrated graticule, scale bar or magnification with consistent units
an actual dimension
What kind of cell is it?
scale, nucleus or nucleoid, organelles, wall material and context
prokaryotic or a eukaryotic lineage
What does a structure do?
visible geometry connected to a known mechanism
a defensible structure–function inference
A strong conclusion keeps observation → interpretation → limitation in that order. One clue may suggest an identity; several independent clues make it defensible.
Cells as basic structural unit
[ 2 ]
Outline the cell theory.
Microscopy skills
[ 3 ]
Outline the procedure for focusing a light microscope.
Developments in microscopy
[ 1 ]
What is a feature of immunofluorescence in light microscopy?
Structures common to all cells
[ 2 ]
List two structures that neurons have in common with prokaryotic cells.
Prokaryote cell structure
[ 9 ]
Escherichia coli is a unicellular organism, so each cell must carry out all of the processes required for life. Outline the functions of each of the structures in the cells of Escherichia coli.
Drawing canvas
Eukaryote cell structure
[ 2 ]
Label structures I, II, III and IV. I. II. III. IV.
Drawing canvas
Processes of life in unicellular organisms
[ 4 ]
Unicellular and multicellular organisms share the same functions of life. Outline four functions of life.
Differences in eukaryotic cells
[ 3 ]
Distinguish between structures in animal and plant cells.
Atypical cell structure
[ 7 ]
Discuss the cell theory and its limitations.
Cell identification in micrographs
[ 3 ]
Identify organelles I to III.
I: II: III:
Drawing and annotation
[ 3 ]
Draw a labelled diagram of a nucleus from a eukaryotic cell, such as an onion epidermis cell, as seen using an electron microscope.
Drawing canvas
A3.1 Diversity of organisms
Diversity of organisms links variation, species concepts, classification tools, chromosome evidence, and genome comparisons to explain biological similarity and difference.
Syllabus
First assessment 2025
Topic
A3.1
Level
SL
Variation is the pattern that selection can act on
Pattern
What the data look like
Common biological basis
Continuous variation
A measured range with intermediate values
Often many genes plus environmental effects
Discontinuous variation
Distinct categories with no intermediates
Often one or a few genes with large effects
Variation occurs among individuals in every species. Darwin recognized that, when some inherited variants affect survival or reproduction, their frequencies can change across generations by natural selection.
Morphology is useful evidence, not an automatic verdict
The morphological species concept groups organisms by a shared set of diagnostic structures. It is fast in fieldwork and can be applied to fossils, plants and asexual organisms when breeding evidence is unavailable.
Appearance varies within species. Sexual dimorphism can make males and females look unlike, while unrelated species can converge on similar forms. A boundary should therefore use several traits and, where possible, independent evidence.
A binomial gives every species one universal name
A scientific species name has two parts: the genus followed by the specific epithet. In Panthera leo, Panthera names the genus and leo distinguishes the species within that genus.
Capitalize the genus; write the specific epithet in lowercase.
Italicize both words when typed; underline each word separately when handwritten.
After the first full use, the genus may be abbreviated: P. leo.
The shared genus indicates a group of similar, closely related species. The binomial prevents ambiguity between local common names, but the name records a classification—it is not itself evidence that the species boundary is correct.
Gene flow defines the biological species concept
Under the biological species concept, members of one species can interbreed and produce viable, fertile offspring. Successful reproduction lets alleles move through a shared gene pool.
Question
What failure would show
Can mating and fertilization occur?
A pre-zygotic reproductive barrier
Does the hybrid survive?
Reduced hybrid viability
Can the hybrid produce functional gametes?
Hybrid sterility
This concept cannot be tested directly for fossils, and it does not fit organisms that reproduce only asexually. Distinct forms may also retain limited gene flow, so interbreeding evidence must be interpreted with ecology and evolutionary history.
A gradual process creates a fuzzy species boundary
1
Populations begin within one species and exchange genes, so their members remain genetically connected.
2
A geographic or ecological barrier reduces gene flow. Mutation, selection and genetic drift can then change the populations independently.
3
Differences accumulate gradually; there is no single moment at which every trait changes from ‘same species’ to ‘different species’.
4
Separated populations may still be theoretically capable of interbreeding even though they never meet. Scientists therefore infer a boundary from converging reproductive, morphological, ecological and genetic evidence.
Chromosome number is characteristic, not a complexity scale
Species
Diploid chromosome number
Fruit fly
8
Rice
24
Human
46
Chimpanzee
48
Dog
78
In a typical diploid cell, chromosomes occur as homologous pairs—one member of each pair inherited from each parent—so the diploid number is usually even and normally constant within a species.
Chromosome number varies widely between species but does not rank organism size or complexity. Closely related species can differ by a chromosome fusion or split while retaining many homologous genes.
A karyogram turns chromosome structure into evidence
A karyotype is the chromosome number and structural features of a cell. A karyogram is an arranged image in which homologous chromosomes are paired using length, banding pattern and centromere position.
Three independent matches support ancestral fusion: human chromosome 2 follows the combined band order of chimpanzee 2A and 2B, contains telomere-like sequence internally, and retains a vestigial second centromere. Together these are stronger than chromosome count alone.
One species combines genomic unity with variant sites
A genome is all genetic information in an organism: nuclear DNA plus mitochondrial DNA and, in plants, chloroplast DNA. Messenger RNA and transfer RNA are products of genes, not additional parts of the genome.
Scale
Pattern
Meaning
Most sites within one species
The same base sequence is shared
Common ancestry and shared biological organization
Variant sites
Alleles differ among individuals
Heritable diversity within the species
Single-nucleotide polymorphism (SNP)
One base differs at a particular position
A marker that can be tracked through populations or families
Most SNPs are in non-coding DNA and have no known phenotypic effect. Some lie in genes or regulatory regions and can affect function, drug response or disease risk; association alone does not prove causation.
Genome size and genome sequence answer different questions
Measure
What it records
What it does not tell you alone
Base sequence
The order of nucleotides
Whether every difference changes phenotype
Genome size
DNA in one haploid chromosome set
Gene number or organism complexity
Eukaryote genomes vary in both sequence and total DNA. Sequence variation between species is much larger than variation within one species, while repeated and other non-coding DNA can contribute greatly to genome size.
Whole-genome duplication produces polyploid organisms with several chromosome sets. This can greatly enlarge plant genomes without making the organism proportionally more complex, so a genome-size comparison is not a complexity ranking.
Sequencing produces a dataset; validation produces a conclusion
Whole-genome sequencing determines the order of bases across an organism’s complete DNA. The Human Genome Project established large-scale reference sequencing and accelerated bioinformatics for storing, comparing and interpreting sequence data.
Current and developing uses include:
comparing evolutionary relationships
locating genes and variants associated with disease
identifying and tracking pathogens
finding possible diagnostic, treatment or vaccine targets
guiding prevention or treatment using an individual’s genetic profile
A sequence match or statistical association generates a hypothesis. Clinical effect, causation and safety require independent evidence; sequencing alone does not diagnose every disease or guarantee that a proposed target will work.
Summary: match the species question to the evidence
Question
Useful evidence
Main limit
How do individuals vary?
Trait distributions, genes and environment
A pattern does not identify its cause by itself
Where is a species boundary?
Morphology, gene flow and fertile offspring
Speciation is gradual and some cases cannot be crossed or observed
What changed in chromosome history?
Number, banding, centromeres and sequence landmarks
One similarity alone is weak evidence
How do genomes differ?
Sequence variants and genome size measured at the right scale
More DNA does not mean greater complexity
Start with the biological question, choose evidence at the organism, reproductive, chromosome or sequence scale, then state what that evidence cannot decide alone.
Variation as defining feature of life
[ 2 ]
State, giving a reason, the type of variation shown by shell length.
Species as groups with shared traits
[ 2 ]
List two anatomical features of humans that are characteristic of primates. 1. 2.
Binomial system
[ 4 ]
Outline the binomial system of classification.
Biological species concept
[ 3 ]
Outline the criteria that should be used to assess whether a group of organisms is a species.
Difficulties in distinguishing species
[ 1 ]
State with a reason whether the genetic evidence shows that the western coyote and the grey wolf have overlapping ranges.
Diversity in chromosome numbers
[ 1 ]
State the chromosome number in this gorilla.
Karyotyping and karyograms
[ 8 ]
Explain the use of karyotyping in human genetics.
Drawing canvas
Unity and diversity of genomes within
[ 7 ]
Discuss the role of genes and chromosomes in determining individual and shared character features of the members of a species.
Diversity of eukaryote genomes
[ 1 ]
The table shows the estimated total number of genes in several organisms.
Species
Estimated number of genes
Saccharomyces cerevisiae (a yeast)
6000
Escherichia coli (a bacterium)
3200
Drosophila melanogaster (fruit fly)
14000
Canis familiaris (domestic dog)
19000
Oryza sativa (rice)
51000
Homo sapiens (human)
25000
What can be deduced from the information in this table?
Comparison of genome sizes
[ 2 ]
Using the data provided in the table, discuss whether genome size positively correlates to organism complexity.
Whole genome sequencing
[ 3 ]
Discuss the current and potential future uses of whole genome sequencing.
A4.1 Evolution and speciation
Evolution and speciation explain how heritable variation, molecular evidence, selection, isolation, and chromosome change produce population divergence and new species over time.
Syllabus
First assessment 2025
Topic
A4.1
Level
SL
Evolution changes populations across generations
Evolution is the cumulative change in the heritable characteristics of a population across generations. The population evolves when inherited variants change in frequency; an individual does not evolve during its lifetime.
Change
Inherited by offspring?
Evolutionary meaning
A DNA variant in a reproductive lineage
potentially
can enter the population gene pool
A stable heritable change in gene regulation
sometimes
can contribute while it remains transmissible
Larger muscles from training or a stretched body part
no
an acquired phenotype, not Darwinian evolution
Mutation and recombination generate heritable variation. Selection, genetic drift and gene flow alter which variants are passed on, so the distribution of characteristics in the next generations can differ from the starting population.
Molecular unity and difference reveal evolutionary history
Nearly all cellular life stores heredity in DNA, uses an almost universal genetic code, translates RNA on ribosomes, transfers energy with ATP and shares core metabolic chemistry. This deep unity is evidence of common ancestry.
Observation in homologous sequences
Supported inference
many conserved positions
inheritance from a shared ancestor
fewer sequence differences between two taxa
usually a more recent common ancestor
differences accumulated after a split
evidence of divergence, interpreted with the gene and model used
Sequence similarity is evidence, not a complete history by itself. The sequences must be homologous, sampling must be adequate, and different genes or evolutionary models can yield different estimates.
Selective breeding exposes the power of inherited variation
1
A breeding population already contains heritable variation produced by mutation and recombination. The breeder does not create the desired allele by wanting it.
2
Individuals with a desired phenotype are chosen as parents, so alleles contributing to that phenotype are overrepresented among their offspring.
3
Repeating the choice over generations shifts the population mean and can produce striking breeds or crop forms, as in pigeons, Brassica vegetables and maize.
Artificial and natural selection act on inherited variation, but the selecting agent differs: human preference in breeding, and differential survival or reproduction in the environment.
Homology records divergence; analogy records convergence
Pattern
Origin
Evolutionary process
Example
Homologous structures
one ancestral structure, modified for different functions
divergent evolution
pentadactyl forelimbs for grasping, flight, running or swimming
Analogous structures
independent structures shaped by similar selection
convergent evolution
bat and insect wings as flight surfaces
Similarity of function does not establish close ancestry. Classify evolutionary relationships using homologous features and independent molecular evidence; use analogous features to explain how similar niches can produce similar solutions.
Speciation begins when gene flow no longer unifies a population
1
One ancestral species begins as populations exchanging alleles through reproduction; gene flow tends to keep their gene pools similar.
2
A geographic, ecological, temporal, behavioural or chromosome barrier reduces allele exchange, so the populations no longer evolve as one fully connected gene pool.
3
Mutation, genetic drift and different selection pressures alter allele frequencies independently on each side of the barrier.
4
If reproductive isolation becomes strong enough that the lineages no longer exchange genes and produce fertile offspring, one pre-existing species has split into two or more species.
Isolation changes which variants succeed in each gene pool
1
Chimpanzees occur mainly north of the Congo River and bonobos south of it. A founder population crossing during a lower-water period, followed by renewed river isolation, is one evidence-based explanation for their divergence from a common ancestor.
2
The river restricts movement and mating, so alleles are not continually mixed between the populations. Isolation creates the conditions for divergence; it does not directly specify which traits will evolve.
3
Different environments and social or ecological interactions can favour different heritable variants, while mutation and genetic drift also change each gene pool independently. Over many generations these processes can establish reproductive isolation.
A present-day barrier and a modern distribution support a historical hypothesis but do not by themselves date the split. Fossil, geological and molecular evidence are needed to test when and how isolation occurred.
Summary: match evolutionary evidence to the claim
Evidence
What it supports
What it does not show alone
molecular universals
deep common ancestry
the exact branching history
homologous-sequence differences
relative relatedness and divergence
a conclusion independent of gene choice or model
selective breeding
populations respond rapidly to selection on heritable variation
that humans direct natural evolution
homologous structures
descent with modification
identical function
analogous structures
convergence under similar selection
recent common ancestry
Evolution can change one connected population without creating a new species. Speciation specifically requires a pre-existing species to split: gene flow falls, gene pools diverge independently, and reproductive isolation prevents renewed allele mixing.
Keep the scale explicit: individuals carry variants and reproduce; populations evolve; lineages split into species.
Evolution as change in heritable characteristics
[ 8 ]
Explain how the process of evolution occurs.
Evidence from sequences
[ 6 ]
Discuss how variations in proteins can indicate phylogeny and be used as an evolutionary clock.
Evidence from selective breeding
[ 3 ]
Outline the evidence for evolution provided by selective breeding.
Evidence from homologous structures
[ 4 ]
Describe the evidence for evolution from homologous structures.
Convergent evolution
[ 4 ]
Explain how analogous structures can evolve.
Speciation by splitting of pre-existing species
[ 5 ]
Describe the changes that occur in gene pools during speciation.
Reproductive isolation and differential selection
[ 7 ]
Explain how isolation leads to speciation.
A4.2 Conservation of biodiversity
Conservation of biodiversity connects biodiversity measurement, human-driven losses, ecosystem evidence, and targeted conservation strategies for protecting species, communities, and evolutionary history.
Syllabus
First assessment 2025
Topic
A4.2
Level
SL
Biodiversity Exists at Three Linked Levels
Biodiversity is the variety of life at genetic, species and ecosystem levels. A community can have the same richness as another yet lower diversity if one species dominates.
D=∑n(n−1)N(N−1)
N is the total number of organisms and n is the number in each species. For comparable samples, a larger D indicates greater species diversity.
Present and Past Biodiversity Need Different Evidence
Present biodiversity is estimated from field surveys, species records and DNA-based discovery. Past biodiversity is reconstructed from fossils placed in dated geological contexts.
Claim
Main evidence
Main limit
about 9 million living species
sampled habitats, described species and extrapolation
many habitats and species remain unsampled or undescribed
change in diversity through geological time
fossils, stratigraphy and radiometric dating
fossilisation and discovery are selective and incomplete
Trace Human Pressure to Extinction through Populations
Human activity can raise mortality, remove habitat and shrink populations until recovery is impossible. Small fragmented populations also face inbreeding, genetic drift and chance events.
Case
Human pressure
Population effect
Wider effect
North Island giant moa
hunting and forest clearance
mortality rose while habitat fell
extinction
Caribbean monk seal
hunting and depletion of food by fishing
a small population could not recover
extinction and loss of a top predator
Ecosystem Loss Breaks Structure and Process
Ecosystem loss includes conversion, degradation and fragmentation. These change habitat structure and abiotic conditions, disrupt interactions and nutrient cycling, and reduce resilience.
Ecosystem
Pressures
Biological pathway
Borneo mixed dipterocarp forest
logging, fire and oil-palm conversion
canopy and habitat loss → fragmentation → decline of forest communities
Great Barrier Reef
warming, falling pH, runoff and fishing
coral stress and mortality → habitat loss → reef-community decline
A Biodiversity Crisis Is a Trend, Not One Snapshot
A biodiversity crisis is supported by consistent declines across repeated, comparable measurements, not by one low count.
Set a baseline with defined sites, season, method and sampling effort.
Repeat the same protocol and control changes in detectability.
Track richness, evenness, abundance and, where suitable, Simpson reciprocal index.
Compare independent datasets and syntheses such as IPBES reports.
Evidence source
Strength
Reliability check
peer-reviewed study
detailed methods and scrutiny
sampling design, replication and uncertainty
citizen science
wide spatial and temporal coverage
training, validation and correction for reporting bias
Human Demand Acts through Several Biological Drivers
Population size and consumption create pressure through land, food, energy and material demand. Biodiversity changes through the biological mechanism of each driver.
Driver
Biological mechanism
Useful response to monitor
habitat conversion and urbanisation
habitat area and connectivity fall
range, occupancy and fragment connectivity
overexploitation
mortality exceeds recruitment
abundance, age structure and harvest rate
pollution and climate change
survival, reproduction or species interactions change
reproductive success, mortality and distribution
introduced invasive species
competition, predation, disease or habitat change
native abundance and invader spread
An alien species occurs outside its natural range through human activity. It is invasive when it spreads and causes ecological or economic harm; alien does not automatically mean invasive.
Conservation Must Match the Threat and the Scale
Conservation works when the intervention removes or reduces the diagnosed threat and protects a viable population, habitat or ecological process.
Approach
Best suited to
Examples
Key condition
in situ
protecting species in functioning habitat
reserves, corridors, rewilding
threats must be controlled in the habitat
ex situ
preventing immediate loss or preserving genetic material
captive breeding, zoos, botanic gardens, seed and tissue banks
genetic diversity and suitable release habitat must be maintained
diagnose threat → select action at the same scale → set a measurable biological target → monitor → adapt management
EDGE Prioritizes Unique Evolutionary History at Risk
EDGE combines Evolutionary Distinctiveness (ED) with Global Endangerment (GE). ED measures how isolated a species is on the tree of life; GE reflects IUCN extinction risk.
High ED + high GE → high EDGE priority. Protecting such a species avoids losing a disproportionately large amount of evolutionary history.
EDGE is a prioritisation tool, not the whole conservation plan: feasibility, ecosystem role, cost and the causes of decline still shape the intervention.
Summary: Turn Biodiversity Evidence into Action
Focus
Measure the state or trend
Diagnose the driver
Match the action
Monitor recovery
genetic diversity
allelic variation, heterozygosity, population size
isolation, bottleneck or inbreeding
reconnect populations or manage breeding
genetic diversity and effective population size
species diversity
richness, evenness, abundance, extinction risk
exploitation, invasive species or habitat change
protect, control threats, breed or reintroduce
abundance, recruitment and distribution
ecosystem diversity/function
habitat extent, condition and process indicators
conversion, fragmentation, pollution or climate stress
protect, restore and reconnect habitat
structure, processes and resilience
Define the level → establish a comparable trend → identify the causal driver → choose an action at the same scale → test whether biological recovery follows.
No single index or intervention is sufficient: species counts can miss evenness, genetic erosion and ecosystem dysfunction, while a reserve can fail without connectivity, enforcement or threat removal.
Biodiversity definition
[ 4 ]
Entire communities need to be conserved in order to preserve biodiversity.
Suggest different quantitative methods to measure changes in biodiversity in a community over time.
Anthropogenic species extinction
[ 1 ]
The North Island giant moa (Dinornis novaezealandiae) went extinct some time between the arrival of humans in New Zealand and the 1800s.
What do scientists believe to be the cause of this extinction?
Ecosystem loss causes
[ 1 ]
The satellite images show changes in the area covered by forest, much of which is mixed dipterocarp forest, on the island of Borneo in Southeast Asia.
Which human activities are most likely to have caused the changes shown in the map?
Causes of biodiversity crisis
[ 3 ]
List human actions that could threaten populations of insect pollinators such as bees.
Conservation approaches
[ 4 ]
Discuss in situ and ex situ conservation of endangered species.
EDGE of Existence programme
[ 2 ]
Outline the importance of the EDGE of Existence programme.