A4.1.7—Reproductive isolation and differential selection

Reproductive isolation stops gene flow, allowing different selection pressures and genetic changes to drive populations along separate evolutionary paths toward speciation.

Syllabus
First assessment 2025
Objective
A4.1.7
Level
HL

Exam analysis

Chance of appearing4%of analysed past papers
Latest appearanceMay 2024
Most common paperPaper1
Typical marks1–3

Common command terms

  • Identify
  • Analyse
  • State
  • Describe
  • Outline
  • Distinguish
  • Explain

Scoring notes

Common mistake
Listing a barrier but not connecting it to reduced gene flow.

Recent exam appearances

May 2024Paper2 ["HL"] · TZ17(c)[ 7 ]A4.1.7—Reproductive isolation and differential selection
November 2022Paper1 ["HL"] · TZ036[ 1 ]A4.1.7—Reproductive isolation and differential selection
May 2018Paper1 ["HL"] · TZ117[ 1 ]A4.1.7—Reproductive isolation and differential selection
May 2017Paper2 ["HL"] · TZ27(a)[ 3 ]A4.1.7—Reproductive isolation and differential selection
November 2012Paper3 ["HL"] · TZ0D2(a)(ii)[ 1 ]A4.1.7—Reproductive isolation and differential selection
Practice this objective

Coverage 2012–2024 · Updated 15 Jul 2026

Trace Species Splitting from Isolation to Divergence

Gene flow reduction and divergence.

Speciation is the splitting of one pre-existing species into two or more species; it increases the total number of species, whereas extinction decreases it.

Reduced gene flow lets separated gene pools diverge. Mutation supplies variation, differential selection changes allele frequencies under different conditions, and drift can add chance divergence until reproductive isolation prevents fertile gene flow.

Geographic isolation can begin the process. The Congo River separated populations ancestral to bonobos and common chimpanzees; different conditions on either side supported differential selection and divergence.

If two isolated populations accumulate different mating traits and eventually cannot produce fertile offspring when reunited, one ancestral species has split into two.

Geographic separation alone is not speciation, and gradual evolutionary change within one continuing lineage does not increase species number. The defining outcome is reproductive isolation after splitting.

Reproductive isolation and differential selection

Assessment in practice

1–3 marks
How it is assessed

This objective is assessed through structured response, commonly using Identify / Analyse / State.

Command terms

Identify / Analyse / State / Describe / Outline / Distinguish / Explain

What earns marks

Build the answer around this relationship: Reproductive isolation reduces or prevents gene flow between populations.

Watch for

Listing a barrier but not connecting it to reduced gene flow.

Representative question

Question 1

[Maximum number: 7]

Explain how isolation leads to speciation.

SL Transfer: Evidence To Speciation

The core A4.1 answer moves from evidence to mechanism. Define evolution as heritable population change across generations. Use molecular universals and sequence differences, selective breeding, homology, and convergence as evidence for common ancestry and divergence. Then explain speciation as reduced gene flow followed by divergence of isolated gene pools through selection, mutation, and drift.

  • Evolution is cumulative change in heritable characteristics of a population.
  • Molecular evidence supports common ancestry and divergence.
  • Selective breeding shows heritable traits can change rapidly under selection.
  • Homology supports common ancestry; analogy/convergence shows similar selection pressures can mislead.
  • Speciation needs reduced gene flow and divergence of isolated gene pools.

Concept essentials

  • Reproductive isolation reduces or prevents gene flow between populations.
  • Temporal, behavioural and geographic barriers can separate gene pools.
  • Different selection pressures can favour different adaptations in isolated populations.
  • Speciation is supported when divergence prevents successful fertile interbreeding.