A4.1.6—Speciation by splitting of pre-existing species

Speciation occurs when a pre-existing species splits into isolated gene pools that diverge until successful interbreeding is no longer possible.

Syllabus
First assessment 2025
Objective
A4.1.6
Level
HL

Exam analysis

Chance of appearing6%of analysed past papers
Latest appearanceMay 2025
Most common paperPaper2
Typical marks2–7

Common command terms

  • Discuss
  • Outline
  • Suggest
  • Explain
  • Describe

Scoring notes

Common mistake
Naming geographic separation without explaining gene-pool divergence or reproductive isolation.

Recent exam appearances

May 2025Paper1A ["HL"] · TZ221[ 1 ]A4.1.6—Speciation by splitting of pre-existing species
May 2022Paper2 ["HL"] · TZ27(c)[ 7 ]A4.1.6—Speciation by splitting of pre-existing species
November 2020Paper2 ["HL"] · TZ08(b)[ 5 ]A4.1.6—Speciation by splitting of pre-existing species
May 2019Paper2 ["HL"] · TZ17(b)[ 7 ]A4.1.6—Speciation by splitting of pre-existing species
May 2017Paper2 ["HL"] · TZ17(b)[ 4 ]A4.1.6—Speciation by splitting of pre-existing species
Practice this objective

Coverage 2013–2025 · Updated 15 Jul 2026

Trace Species Splitting from Isolation to Divergence

Gene flow reduction and divergence.

Speciation is the splitting of one pre-existing species into two or more species; it increases the total number of species, whereas extinction decreases it.

Reduced gene flow lets separated gene pools diverge. Mutation supplies variation, differential selection changes allele frequencies under different conditions, and drift can add chance divergence until reproductive isolation prevents fertile gene flow.

Geographic isolation can begin the process. The Congo River separated populations ancestral to bonobos and common chimpanzees; different conditions on either side supported differential selection and divergence.

If two isolated populations accumulate different mating traits and eventually cannot produce fertile offspring when reunited, one ancestral species has split into two.

Geographic separation alone is not speciation, and gradual evolutionary change within one continuing lineage does not increase species number. The defining outcome is reproductive isolation after splitting.

Speciation by splitting of pre-existing species

Assessment in practice

2–5 marks
How it is assessed

This objective is assessed through structured response, commonly using Discuss / Outline / Suggest.

Command terms

Discuss / Outline / Suggest / Explain / Describe

What earns marks

Build the answer around this relationship: Speciation forms new species by splitting a pre-existing species into diverging populations.

Watch for

Naming geographic separation without explaining gene-pool divergence or reproductive isolation.

Representative question

Question 1

[Maximum number: 5]

Describe the changes that occur in gene pools during speciation.

SL Transfer: Evidence To Speciation

The core A4.1 answer moves from evidence to mechanism. Define evolution as heritable population change across generations. Use molecular universals and sequence differences, selective breeding, homology, and convergence as evidence for common ancestry and divergence. Then explain speciation as reduced gene flow followed by divergence of isolated gene pools through selection, mutation, and drift.

  • Evolution is cumulative change in heritable characteristics of a population.
  • Molecular evidence supports common ancestry and divergence.
  • Selective breeding shows heritable traits can change rapidly under selection.
  • Homology supports common ancestry; analogy/convergence shows similar selection pressures can mislead.
  • Speciation needs reduced gene flow and divergence of isolated gene pools.

Concept essentials

  • Speciation forms new species by splitting a pre-existing species into diverging populations.
  • Separated gene pools can diverge through mutation, drift and different selection pressures.
  • Reproductive isolation means gene flow is reduced or absent between populations.
  • A biological species boundary exists when fertile offspring cannot be produced by interbreeding.