A4.1.6—Speciation by splitting of pre-existing species
Speciation occurs when a pre-existing species splits into isolated gene pools that diverge until successful interbreeding is no longer possible.
Syllabus
First assessment 2025
Objective
A4.1.6
Level
HL
Exam analysis
Chance of appearing6%of analysed past papers
Latest appearanceMay 2025
Most common paperPaper2
Typical marks2–7
Common command terms
Discuss
Outline
Suggest
Explain
Describe
Scoring notes
Common mistake
Naming geographic separation without explaining gene-pool divergence or reproductive isolation.
Recent exam appearances
SessionPaperQuestionMarksFocus
May 2025Paper1A ["HL"] · TZ221[ 1 ]A4.1.6—Speciation by splitting of pre-existing species
May 2022Paper2 ["HL"] · TZ27(c)[ 7 ]A4.1.6—Speciation by splitting of pre-existing species
November 2020Paper2 ["HL"] · TZ08(b)[ 5 ]A4.1.6—Speciation by splitting of pre-existing species
May 2019Paper2 ["HL"] · TZ17(b)[ 7 ]A4.1.6—Speciation by splitting of pre-existing species
May 2017Paper2 ["HL"] · TZ17(b)[ 4 ]A4.1.6—Speciation by splitting of pre-existing species
Practice this objective
Coverage 2013–2025 · Updated 15 Jul 2026
Trace Species Splitting from Isolation to Divergence
Speciation is the splitting of one pre-existing species into two or more species; it increases the total number of species, whereas extinction decreases it.
Reduced gene flow lets separated gene pools diverge. Mutation supplies variation, differential selection changes allele frequencies under different conditions, and drift can add chance divergence until reproductive isolation prevents fertile gene flow.
Geographic isolation can begin the process. The Congo River separated populations ancestral to bonobos and common chimpanzees; different conditions on either side supported differential selection and divergence.
If two isolated populations accumulate different mating traits and eventually cannot produce fertile offspring when reunited, one ancestral species has split into two.
Geographic separation alone is not speciation, and gradual evolutionary change within one continuing lineage does not increase species number. The defining outcome is reproductive isolation after splitting.
Speciation by splitting of pre-existing species
Assessment in practice
2–5 marks
How it is assessed
This objective is assessed through structured response, commonly using Discuss / Outline / Suggest.
Command terms
Discuss / Outline / Suggest / Explain / Describe
What earns marks
Build the answer around this relationship: Speciation forms new species by splitting a pre-existing species into diverging populations.
Watch for
Naming geographic separation without explaining gene-pool divergence or reproductive isolation.
Representative question
Question 1
[Maximum number: 5]
Describe the changes that occur in gene pools during speciation.
a gene pool is all genes/alleles in an (interbreeding) population;
b gene pool splits/divides/separated during speciation;
c due to reproductive isolation (of groups within a species);
d temporal/behavioral/geographic isolation (can cause reproductive isolation);
e divergence of gene pools;
f allele frequencies change;
g natural selection different (in the isolated groups so there is divergence);
h different (random) mutations occur (in the isolated populations so there is divergence);
i speciation has occurred when differences between populations prevent interbreeding;
Marking guidance:
Do not award both mpc and mpi for the same idea (reproductive isolation separating populations vs speciation due to interbreeding not being possible)
5 max
SL Transfer: Evidence To Speciation
The core A4.1 answer moves from evidence to mechanism. Define evolution as heritable population change across generations. Use molecular universals and sequence differences, selective breeding, homology, and convergence as evidence for common ancestry and divergence. Then explain speciation as reduced gene flow followed by divergence of isolated gene pools through selection, mutation, and drift.
Evolution is cumulative change in heritable characteristics of a population.
Molecular evidence supports common ancestry and divergence.
Selective breeding shows heritable traits can change rapidly under selection.
Homology supports common ancestry; analogy/convergence shows similar selection pressures can mislead.
Speciation needs reduced gene flow and divergence of isolated gene pools.
Concept essentials
Speciation forms new species by splitting a pre-existing species into diverging populations.
Separated gene pools can diverge through mutation, drift and different selection pressures.
Reproductive isolation means gene flow is reduced or absent between populations.
A biological species boundary exists when fertile offspring cannot be produced by interbreeding.