B3.1 Gas exchange
Gas exchange links animal ventilation, alveolar diffusion, leaf stomata, transpiration and haemoglobin affinity to oxygen supply and carbon dioxide removal.
- Syllabus
- First assessment 2025
- Topic
- B3.1
- Level
- SL
Gas exchange links animal ventilation, alveolar diffusion, leaf stomata, transpiration and haemoglobin affinity to oxygen supply and carbon dioxide removal.

Coverage 2010–2025 · Updated 15 Jul 2026
• Gas exchange supplies respiratory gases between organisms and environment
• Animals take in oxygen and release carbon dioxide for aerobic respiration
• Larger or active organisms need specialized exchange surfaces because SA:V decreases
• Efficient gas-exchange surfaces are permeable, thin, moist, and large
• Large surface area and steep concentration gradients increase diffusion
• Short diffusion paths speed movement of oxygen and carbon dioxide
• Dense capillary networks provide large exchange area and rapid transport
• Continuous blood flow carries oxygen away and carbon dioxide toward the surface
• Ventilation refreshes air or water to maintain steep gas gradients
• Many small alveoli provide large surface area and thin squamous walls
• Capillary beds maintain gradients and bring red blood cells close to alveolar air
• Surfactant reduces surface tension, while elastic fibres support ventilation and recoil
• Diaphragm and intercostal muscles change thoracic volume and pressure
• Inspiration uses diaphragm and external intercostal contraction to draw air in
• Expiration reduces thoracic volume; internal intercostals and abdominal muscles aid forced exhalation
• Spirometry records breathing patterns, ventilation rate, and lung volumes
• Tidal volume is air moved during normal relaxed breathing
• Vital capacity equals tidal volume plus inspiratory and expiratory reserve volumes
• Stomata allow carbon dioxide, oxygen, and water vapour to diffuse
• Guard cells open and close stomata by changes in turgor
• Waxy cuticle, lower epidermal stomata, air spaces, mesophyll, and veins balance gas exchange with water loss
• Dicot leaves have waxy cuticle, upper and lower epidermis, palisade and spongy mesophyll
• Vascular bundles contain xylem and phloem for water and assimilate transport
• Spongy mesophyll air spaces and stomata create internal diffusion routes
• Transpiration is evaporation from mesophyll walls followed by diffusion of water vapour through stomata
• Temperature, humidity, wind, and light alter concentration gradients and stomatal opening
• Potometers estimate transpiration indirectly by measuring water uptake
• Stomatal density is the number of stomata per unit leaf area
• Higher density can increase carbon dioxide uptake and water loss
• Leaf casts or micrographs allow stomatal counts and comparisons between species