CAIE A-Level Biology AS 3.2.2 Vmax and Km Questions

Interpret Vmax and Km as measures of enzyme kinetics using the reaction-rate evidence in each question.

Exam points

Explain Vmax and Km and interpret their meaning in enzyme-catalysed reaction-rate investigations.

[Maximum number: 1]

Which aspect of enzyme activity can be compared by the Michaelis-Menten constant?

activation energy of a reaction with or without an enzyme

affinity of different enzymes for their substrates

affinity of an enzyme at different substrate concentrations

maximum rate of reaction ( $\mathrm{V}_{\text {max }}$ ) at different temperatures

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